Ecotoxicology - characterisation of the branch; definition of ecotoxicological biotests, their types according to the following criteria: exposure time, level of design of testing system, trophic level of testing systems, matrix tested, type of tested sample, method of evaluation; the importace and purpose of toxicity tests with various substances and preparations. Legislation dealing with ecotoxicological issues in the Czech Republic. Ecotoxicity - methods of determination. Waste ecotoxicity, classes of leaching status, LC (EC,IC) 50, testing scheme; limit, preliminary, basic, introductory, control tests; animals used for ecotoxicological tests. Evaluation of toxicity of substances and wastes for various organisms including the terrestric ones. Tests performed on higher plants and fungi - phytotoxicity tests.