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FCH-MC_MOB_PAcad. year: 2013/2014
Safety of work in microbiological and biotechnological laboratory. Arrangement of PCR laboratory. Cultivation of Lactobacillus acidophilus cells for isolation of chromosomal DNA. Cell lysis. Removal of proteins using phenol extraction. Precipitation of DNA with ethanol. Verification of DNA integrity using agarose gel electrophoresis. Spectrophotometric estimation of concentration and purity verification of DNA. Preparation of PCR mixture for amplification of Lactobacillus acidophilus using purified DNA as DNA matrix. Negative controls. PCR amplification. Detection of PCR products using agarose gel electrophoresis. Estimation of length of amplified fragment using external DNA standards. Sensitivity of PCR. Isolation of DNA from dairy product (yoghurt). Identification of Lactobacillus DNA in dairy product (yoghurt) by PCR. Gel electophoresis of amplicons. Test - identifikation of Lactobacillus cells in unknown sample using PCR.
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branch CKCO_CZV , 1 year of study, winter semester, compulsory-optional
branch NPCO_CHTP , 1 year of study, winter semester, compulsory-optional
branch NKCO_CHTP , 1 year of study, winter semester, compulsory-optional
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