Course detail

Bioengineering II

FCH-MC_BIO2Acad. year: 2014/2015

Stechiometry of microbial growth and material balances. Systems with immobilized cells and enzymes. Enzymatic catalysis in anhydrous and quasi anhydrous solvents. Magnetic particles. Micromanipulation of particles. Microflow in capillary tubes. Microdrops. Properties of fluids. Mechanical, chemical and enzymatic cell disruption. Precipitation and salting-out odf proteins. Filtration. Centrifugation. Extraction - types of extractors and their design. Extraction using aqueous two-phase systems. Large scale chromatographic techniques. Production of proteins using genetically modified microorganisms (GMO) and methods of their isolation. Storage of proteins. Validation of production quality.

Language of instruction

Czech

Number of ECTS credits

4

Mode of study

Not applicable.

Learning outcomes of the course unit

Theoretical aspects of various isolation and separation techniques focused on microorganism cells

Prerequisites

Without restriction

Co-requisites

Not applicable.

Planned learning activities and teaching methods

The course uses teaching methods in form of Lecture - 2 teaching hours per week, seminars - 1 teaching hour per week. The e-learning system (LMS Moodle) is available to teachers and students.

Assesment methods and criteria linked to learning outcomes

Oral examination

Course curriculum

1. Stechiometry of microbial growth and material balances.
2. Systems with immobilized cells and enzymes.
3. Enzymatic catalysis in anhydrous and quasi anhydrous solvents.
4. Magnetic particles. Micromanipulation of particles.
5. Microflow in capillary tubes. Microdrops. Properties of fluids.
6. Mechanical, chemical and enzymatic cell disruption.
7. Precipitation and salting-out odf proteins. Filtration. Centrifugation.
8. Extraction - types of extractors and their design.
9. Extraction using aqueous two-phase systems.
10. Large scale chromatographic techniques.
11. Production of proteins using genetically modified microorganisms (GMO) and methods of their isolation.
12. Protein stability.
13. Validation of production quality.

Work placements

Not applicable.

Aims

Lectures cover methods for biotechnoly product processing

Specification of controlled education, way of implementation and compensation for absences

Attendance at lectures is not obligatory but it is recommended. At seminars the calculations of model examples are given - attendance is obligatory.

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Not applicable.

Recommended reading

Not applicable.

Classification of course in study plans

  • Programme NPCP_CHTP Master's

    branch NPCO_CHTP , 1 year of study, winter semester, compulsory

  • Programme NKCP_CHTP Master's

    branch NKCO_CHTP , 1 year of study, winter semester, compulsory

  • Programme CKCP_CZV lifelong learning

    branch CKCO_CZV , 1 year of study, winter semester, compulsory