The aim of the course is to present methods which are used in chemistry, biochemistry and biotechnology in order to separate, characterize, identify and quantify various biomolecules. The presented methods includes centrifugation, dialysis and ultrafiltration. Furthermore, the course will be focused also on chromatography (gel filtration, ion-exchange chromatography, HIC, affinity chromatography) and its utilization for isolation and purification of proteins and other biomolecules. Moreover, attention will be paid also to electrophoretic techniques used for characterization of proteins (PAGE, PAGE-SDS, Tricine-PAGE, native PAGE, 2D electrophoresis etc.) and DNA/RNA (horizontal electrophoresis, DGGE, TGGE etc.). The aim of the course will be also to explain principles of other methods for characterization of molecular mass of biopolymers such as SEC/MALS, ultracentrifugation, MALDI-TOF, DLS and others. In order to understand structure and function of biomolecules, the students will be familiarized with UV-VIS spectrometry, fluorescence spectroscopy, mass spectrometry, microfluidic techniques, flow cytometry and antibody-based assays . The course will also deals with application of optical, fluorescence and electron microscopy to study complex biological systems.