Detail publikace

Investigation of interaction between magnetic silica particles andlambda phage DNA fragment

ŠMERKOVÁ, K. DOSTÁLOVÁ, S. VACULOVIČOVÁ, M. KYNICKÝ, J. TRNKOVÁ, L. KRÁLÍK, M. ADAM, V. HUBÁLEK, J. PROVAZNÍK, I. KIZEK, R.

Originální název

Investigation of interaction between magnetic silica particles andlambda phage DNA fragment

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Nucleic acids belong to the most important molecules and therefore the understanding of theirproperties, function and behavior is crucial. Even though a range of analytical and biochemicalmethods have been developed for this purpose, one common step is essential for all of them –isolation of the nucleic acid from the from complex sample matrix. The use of magnetic parti-cles for the separation of nucleic acids has many advantages over other isolation methods. In thisstudy, an isolation procedure for extraction of DNA was optimized. Each step of the isolation pro-cess including washing, immobilization and elution was optimized and therefore the efficiency wasincreased from 1.7% to 28.7% and the total time was shortened from 75 to 30 min comparing to thepreviously described method. Quantification of the particular parameter influence was performed bysquare-wave voltammetry using hanging drop mercury electrode. Further, we compared the optimizedmethod with standard chloroform extraction and applied on isolation of DNA from Staphylococcus aureusand Escherichia coli.

Klíčová slova

DNA isolation, Magnetic particles, Lambda phage, Polymerase chain reaction, Voltammetry

Autoři

ŠMERKOVÁ, K.; DOSTÁLOVÁ, S.; VACULOVIČOVÁ, M.; KYNICKÝ, J.; TRNKOVÁ, L.; KRÁLÍK, M.; ADAM, V.; HUBÁLEK, J.; PROVAZNÍK, I.; KIZEK, R.

Rok RIV

2013

Vydáno

1. 12. 2013

ISSN

0731-7085

Periodikum

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Ročník

86

Číslo

1

Stát

Nizozemsko

Strany od

65

Strany do

72

Strany počet

13

BibTex

@article{BUT102368,
  author="Kristýna {Šmerková} and Simona {Dostálová} and Markéta {Vaculovičová} and Jindřich {Kynický} and Libuše {Trnková} and Miroslav {Králík} and Vojtěch {Adam} and Jaromír {Hubálek} and Valentine {Provazník} and René {Kizek}",
  title="Investigation of interaction between magnetic silica particles andlambda phage DNA fragment",
  journal="JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS",
  year="2013",
  volume="86",
  number="1",
  pages="65--72",
  doi="10.1016/j.jpba.2013.07.039",
  issn="0731-7085"
}