Detail publikačního výsledku

Interaction of E6 Gene from Human Papilloma Virus 16 (HPV-16) with CdS Quantum Dots

NEJDL, L.; SKALIČKOVÁ, S.; KUDR, J.; RUTTKAY-NEDECKÝ, B.; NGUYEN, H.; MERLOS RODRIGO, M.; KOPEL, P.; NOVOTNÁ, M.; ADAM, V.; KIZEK, R.

Originální název

Interaction of E6 Gene from Human Papilloma Virus 16 (HPV-16) with CdS Quantum Dots

Anglický název

Interaction of E6 Gene from Human Papilloma Virus 16 (HPV-16) with CdS Quantum Dots

Druh

Článek WoS

Originální abstrakt

The aim of this study was to analyze the interactions of blue and yellow fluorescent CdS quantum dots (CdS-QDs) with human papillomavirus 16 (HPV-16) oncogene E6. The interactions were investigated using chip capillary electrophoresis, spectrophotometry and square wave voltammetry (SWV). Using chip capillary electrophoresis we proved that blue fluorescent CdS-QDs (0.5 mM) caused an increase of the migration time of the E6 HPV-16 DNA–CdS-QDs complex by 42 s compared to control DNA (E6 HPV-16). The same concentration of yellow fluorescent CdS-QDs caused an increase in the migration time of the DNA–CdS-QDs complex by 108 s compared to the control DNA (E6 HPV-16). The difference in the migration times between both complexes was 66 s. Using square wave voltammetry (SWV), the reduction signal of cytosine and adenine (peak CA) was observed.

Anglický abstrakt

The aim of this study was to analyze the interactions of blue and yellow fluorescent CdS quantum dots (CdS-QDs) with human papillomavirus 16 (HPV-16) oncogene E6. The interactions were investigated using chip capillary electrophoresis, spectrophotometry and square wave voltammetry (SWV). Using chip capillary electrophoresis we proved that blue fluorescent CdS-QDs (0.5 mM) caused an increase of the migration time of the E6 HPV-16 DNA–CdS-QDs complex by 42 s compared to control DNA (E6 HPV-16). The same concentration of yellow fluorescent CdS-QDs caused an increase in the migration time of the DNA–CdS-QDs complex by 108 s compared to the control DNA (E6 HPV-16). The difference in the migration times between both complexes was 66 s. Using square wave voltammetry (SWV), the reduction signal of cytosine and adenine (peak CA) was observed.

Klíčová slova

Chip capillary electrophoresis, Square wave, voltammetry DNA interaction, human papilloma virus, E6 HPV-16 gene, Cadmium sulphide quantum dots

Klíčová slova v angličtině

Chip capillary electrophoresis, Square wave, voltammetry DNA interaction, human papilloma virus, E6 HPV-16 gene, Cadmium sulphide quantum dots

Autoři

NEJDL, L.; SKALIČKOVÁ, S.; KUDR, J.; RUTTKAY-NEDECKÝ, B.; NGUYEN, H.; MERLOS RODRIGO, M.; KOPEL, P.; NOVOTNÁ, M.; ADAM, V.; KIZEK, R.

Vydáno

01.11.2014

ISSN

0009-5893

Periodikum

CHROMATOGRAPHIA

Svazek

77

Číslo

21-22

Stát

Spolková republika Německo

Strany od

1433

Strany do

1439

Strany počet

7

BibTex

@article{BUT110911,
  author="Lukáš {Nejdl} and Sylvie {Skaličková} and Jiří {Kudr} and Branislav {Ruttkay-Nedecký} and Hoai Viet {Nguyen} and Miguel Ángel {Merlos Rodrigo} and Pavel {Kopel} and Marie {Novotná} and Vojtěch {Adam} and René {Kizek}",
  title="Interaction of E6 Gene from Human Papilloma Virus 16 (HPV-16) with CdS Quantum Dots",
  journal="CHROMATOGRAPHIA",
  year="2014",
  volume="77",
  number="21-22",
  pages="1433--1439",
  doi="10.1007/s10337-014-2734-5",
  issn="0009-5893"
}