Detail publikace

Isolation of Biogenic Amines Using Paramagnetic Microparticles Off-Line Coupled with Ion Exchange Liquid Chromatography

CERNEI, N. HEGER, Z. KOPEL, P. SKLÁDANKA, J. ZÍTKA, O. ADAM, V. KIZEK, R.

Originální název

Isolation of Biogenic Amines Using Paramagnetic Microparticles Off-Line Coupled with Ion Exchange Liquid Chromatography

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

This study aims at the possibility of single structured paramagnetic microparticles (PMPs), composed of maghemite (y-Fe2O3) core modified with chitosan called MAN8, or tetraethyl orthosilicate covered with Dowex called MAN35, to be helpful for isolation of biogenic amines prior to their further analysis. Primarily, we synthesized and characterized PMPs. To obtain the information about bead morphology, scanning electron microscopy was employed. Furthermore, X-ray fluorescence was employed to carry out the elemental composition analyses. To obtain further insight into interaction between PMP surface and biogenic amines, scanning electron microscope was employed. It was shown that binding of biogenic amines causes increase of relative current response of deprotonated microparticles. We tested the specificity of PMPs to bind biogenic amines on histamine, tyramine, spermine, spermidine, putrescine, and cadaverine. We found that two types of our PMPs were able to selectively bind spermidine, cadaverine, and histamine in the case of MAN35; and histamine, tyramine, and putrescine in the case of MAN8. Finally, we carried out the analyses of real samples obtained from patients suffering from prostate carcinoma, where histamine was determined as the most abundant biogenic amine (10.456–13.654 ug mL-1). The prepared PMPs were able to isolate the biogenic amines from real samples, and thus they may be helpful in construction of biosensors, or Lab-on-a-Chip platforms, enabling less painful, and more rapid diagnosis of prostate cancer.

Klíčová slova

Biogenic amines, Biosensor, Ion-exchange liquid chromatography, Magnetic-particles-based separation

Autoři

CERNEI, N.; HEGER, Z.; KOPEL, P.; SKLÁDANKA, J.; ZÍTKA, O.; ADAM, V.; KIZEK, R.

Rok RIV

2014

Vydáno

1. 11. 2014

ISSN

0009-5893

Periodikum

CHROMATOGRAPHIA

Ročník

77

Číslo

21-22

Stát

Spolková republika Německo

Strany od

1451

Strany do

1459

Strany počet

8

BibTex

@article{BUT112435,
  author="Natalia Vladimirovna {Cernei} and Zbyněk {Heger} and Pavel {Kopel} and Jiří {Skládanka} and Ondřej {Zítka} and Vojtěch {Adam} and René {Kizek}",
  title="Isolation of Biogenic Amines Using Paramagnetic Microparticles Off-Line Coupled with Ion Exchange Liquid Chromatography",
  journal="CHROMATOGRAPHIA",
  year="2014",
  volume="77",
  number="21-22",
  pages="1451--1459",
  doi="10.1007/s10337-014-2731-8",
  issn="0009-5893"
}