Quantitative phase imaging through scattering media by means of coherence-controlled holographic microscope

Quantitative phase imaging through scattering media by means of coherence-controlled holographic microscope

Článek WoS

A coherence-controlled holographic microscope (CCHM) enables quantitative phase imaging with coherent as well as incoherent illumination. The low spatially coherent light induces a coherence gating effect, which makes observation of samples possible also through scattering media. The paper describes theoretically and simulates numerically imaging of a two-dimensional object through a static scattering layer by means of CCHM, with the main focus on the quantitative phase imaging quality. The authors have investigated both strongly and weakly scattering media characterized by different amounts of ballistic and diffuse light. It is demonstrated that the phase information can be revealed also for the case of the static, strongly scattering layer. The dependence of the quality of imaging process on the spatial light coherence is demonstrated. The theoretical calculations and numerical simulations are supported by experimental data gained with a model phase object, as well as living carcinoma cells treated in an optically turbid emulsion

A coherence-controlled holographic microscope (CCHM) enables quantitative phase imaging with coherent as well as incoherent illumination. The low spatially coherent light induces a coherence gating effect, which makes observation of samples possible also through scattering media. The paper describes theoretically and simulates numerically imaging of a two-dimensional object through a static scattering layer by means of CCHM, with the main focus on the quantitative phase imaging quality. The authors have investigated both strongly and weakly scattering media characterized by different amounts of ballistic and diffuse light. It is demonstrated that the phase information can be revealed also for the case of the static, strongly scattering layer. The dependence of the quality of imaging process on the spatial light coherence is demonstrated. The theoretical calculations and numerical simulations are supported by experimental data gained with a model phase object, as well as living carcinoma cells treated in an optically turbid emulsion

quantitative phase imaging; digital holography; microscopy; coherence-controlled holographic microscopy; imaging through scattering media; imaging through turbid media.

quantitative phase imaging; digital holography; microscopy; coherence-controlled holographic microscopy; imaging through scattering media; imaging through turbid media.

KOLLÁROVÁ, V.; ČOLLÁKOVÁ, J.; DOSTÁL, Z.; VESELÝ, P.; CHMELÍK, R.

2016

01.11.2015

SPIE

1083-3668

JOURNAL OF BIOMEDICAL OPTICS

20

11

Spojené státy americké

1112016-1

111206-8

8

https://www.spiedigitallibrary.org/journals/Journal-of-Biomedical-Optics/volume-20/issue-11/111206/Quantitative-phase-imaging-through-scattering-media-by-means-of-coherence/10.1117/1.JBO.20.11.111206.full

http://hdl.handle.net/11012/84162

111206_1