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CHMELÍK, R. PETRÁČEK, J. SLABÁ, M. KOLLÁROVÁ, V. SLABÝ, T. ČOLLÁKOVÁ, J. KOMRSKA, J. DOSTÁL, Z. VESELÝ, P.
Originální název
Holographic microscopy in low coherence
Typ
článek ve sborníku ve WoS nebo Scopus
Jazyk
angličtina
Originální abstrakt
Low coherence of the illumination substantially improves the quality of holographic and quantitative phase imaging (QPI) by elimination of the coherence noise and various artefacts and by improving the lateral resolution compared to the coherent holographic microscopy. Attributes of coherence-controlled holographic microscope (CCHM) designed and built as an off-axis holographic system allowing QPI within the range from complete coherent to incoherent illumination confirmed these expected advantages. Low coherence illumination also furnishes the coherence gating which constraints imaging of some spatial frequencies of an object axially thus forming an optical section in the wide sense. In this way the depth discrimination capability of the microscope is introduced at the price of restricting the axial interval of possible numerical refocusing. We describe theoretically these effects for the whole range of illumination coherence. We also show that the axial refocusing constraints can be overcome using advanced mode of imaging based on mutual lateral shift of reference and object image fields in CCHM. Lowering the spatial coherence of illumination means increasing its numerical aperture. We study how this change of the illumination geometry influences 3D objects QPI and especially the interpretation of live cells QPI in terms of the dry mass density measurement. In this way a strong dependence of the imaging process on the light coherence is demonstrated. The theoretical calculations and numerical simulations are supported by experimental data including a chance of time-lapse watching of live cells even in optically turbid milieu.
Klíčová slova
quantitative phase imaging; holographic microscopy; partial coherence; coherence gating; live cells
Autoři
CHMELÍK, R.; PETRÁČEK, J.; SLABÁ, M.; KOLLÁROVÁ, V.; SLABÝ, T.; ČOLLÁKOVÁ, J.; KOMRSKA, J.; DOSTÁL, Z.; VESELÝ, P.
Vydáno
9. 3. 2016
ISBN
978-1-62841-952-8
Kniha
QUANTITATIVE PHASE IMAGING II
ISSN
0277-786X
Periodikum
Proceedings of SPIE
Ročník
9718
Stát
Spojené státy americké
Strany od
971806-1
Strany do
971806-7
Strany počet
7
URL
https://www.spiedigitallibrary.org/conference-proceedings-of-spie/9718/1/Holographic-microscopy-in-low-coherence/10.1117/12.2209465.pdf?SSO=1