Detail publikace

REAL-TIME SENSING OF DOXORUBICIN IN AN ISOLATED RAT HEART

BLAŽKOVÁ, I. VACULOVIČOVÁ, M. NOVÁKOVÁ, M. ADAM, V. KIZEK, R.

Originální název

REAL-TIME SENSING OF DOXORUBICIN IN AN ISOLATED RAT HEART

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

Doxorubixin is a highly effective and widely used antracycline antibiotic cytostatic drug used to treat numerous types of tumour diseases, but the cardiotoxic effect significantly limits its application. Doxorubicin has hot great fluorescence properties what can be used to its detection. The detection of the fluorescence of the therapeutics in organisms is limited by the thickness of the tissue the light need to penetrate. An alternative way for increasing the sensitivity of this type of imaging is the elimination of surrounding tissue, leading to ex vivo analysis under simulated conditions (i.e. perfusion system for isolated heart). The aim of this study was the combination of perfusion system with the fluorescence in vivo imaging system to observe the fluorescence compounds in beating heart. The study proposes the application of in vivo imaging system for fluorescence ex vivo analysis of rat heart from the doxorubicin administered rat. The miniaturized Langendorff perfusion system was used. The isolated heart was supply by oxygenated Tyrode solution (37 °C) to ensure the heart beating and nutrition. This arrangement enabled the detection of doxorubicin in the ex vivo heart. The detection concentration was 1 μg of doxorubicin in the heart after the intraperitoneal application of 4 mg doxorubicin.

Klíčová slova

doxorubicin; fluorescence imaging; langendorff-perfused heart; cancer; magnetic particles

Autoři

BLAŽKOVÁ, I.; VACULOVIČOVÁ, M.; NOVÁKOVÁ, M.; ADAM, V.; KIZEK, R.

Vydáno

21. 11. 2013

ISBN

978-80-7375-908-7

Kniha

MENDELNET 2013

Strany od

691

Strany do

695

Strany počet

5

BibTex

@inproceedings{BUT143941,
  author="Iva {Blažková} and Markéta {Vaculovičová} and Marie {Nováková} and Vojtěch {Adam} and René {Kizek}",
  title="REAL-TIME SENSING OF DOXORUBICIN IN AN ISOLATED RAT HEART",
  booktitle="MENDELNET 2013",
  year="2013",
  pages="691--695",
  isbn="978-80-7375-908-7"
}