Detail publikačního výsledku

ELECTROCHEMICAL DETERMINATION OF LOW MOLECULAR WEIGHT ANTIOXIDANTS IN SERUM SAMPLES

KRACMAROVÁ, A.; SOCHOR, J.; ADAM, V.; KIZEK, R.; POHANKA, M.

Originální název

ELECTROCHEMICAL DETERMINATION OF LOW MOLECULAR WEIGHT ANTIOXIDANTS IN SERUM SAMPLES

Anglický název

ELECTROCHEMICAL DETERMINATION OF LOW MOLECULAR WEIGHT ANTIOXIDANTS IN SERUM SAMPLES

Druh

Stať ve sborníku v databázi WoS či Scopus

Originální abstrakt

The objective of the experiment was to establish and optimize an electrochemical method of low molecular weight antioxidants assay using square wave voltammetry performed on screen printed sensors with carbon working electrodes. The potential range of square wave voltammetry was 0 - 1.1 V. The optimal values for the assay, potential step 5 mV, amplitude 5 mV, frequency 1 Hz and volume of sample 20 µl, were found. The linearity and sensitivity of the method were verified by standard antioxidants - ascorbic acid, trolox, N-acetylcysteine and melatonin. The suitability for low molecular weight antioxidants` assay in human serum samples was confirmed by standard ferric reducing antioxidant power test (FRAP). Limits of detection were 0.3 mmol/l for ascorbic acid, 0.04 mmol/l for melatonin, 0.03 mmol/l for trolox, and 0.07 mmol/l for N-acetylcysteine. There was apparent a strong correlation between square wave voltammetry and the FRAP value as the correlation coefficient was 0.9778.

Anglický abstrakt

The objective of the experiment was to establish and optimize an electrochemical method of low molecular weight antioxidants assay using square wave voltammetry performed on screen printed sensors with carbon working electrodes. The potential range of square wave voltammetry was 0 - 1.1 V. The optimal values for the assay, potential step 5 mV, amplitude 5 mV, frequency 1 Hz and volume of sample 20 µl, were found. The linearity and sensitivity of the method were verified by standard antioxidants - ascorbic acid, trolox, N-acetylcysteine and melatonin. The suitability for low molecular weight antioxidants` assay in human serum samples was confirmed by standard ferric reducing antioxidant power test (FRAP). Limits of detection were 0.3 mmol/l for ascorbic acid, 0.04 mmol/l for melatonin, 0.03 mmol/l for trolox, and 0.07 mmol/l for N-acetylcysteine. There was apparent a strong correlation between square wave voltammetry and the FRAP value as the correlation coefficient was 0.9778.

Klíčová slova

electrochemistry; antioxidant; screen printed sensor; ascorbic acid

Klíčová slova v angličtině

electrochemistry; antioxidant; screen printed sensor; ascorbic acid

Autoři

KRACMAROVÁ, A.; SOCHOR, J.; ADAM, V.; KIZEK, R.; POHANKA, M.

Rok RIV

2018

Vydáno

22.11.2012

ISBN

978-80-7375-836-3

Kniha

MENDELNET 2012

Strany od

1225

Strany do

1232

Strany počet

8

BibTex

@inproceedings{BUT145582,
  author="Alžběta {Kracmarová} and Jiří {Sochor} and Vojtěch {Adam} and René {Kizek} and Miroslav {Pohanka}",
  title="ELECTROCHEMICAL DETERMINATION OF LOW MOLECULAR WEIGHT ANTIOXIDANTS IN SERUM SAMPLES",
  booktitle="MENDELNET 2012",
  year="2012",
  pages="1225--1232",
  isbn="978-80-7375-836-3"
}