Detail publikace

STUDY OF COMPLEXES OF ANIMAL METAL-BINDING PROTEIN WITH PLATINUM CYTOSTATICS

ADAM, P. ZÍTKA, O. KOMÍNKOVÁ, M. SKALIČKOVÁ, S. SKUTKOVÁ, S. ADAM, V. KIZEK, R.

Originální název

STUDY OF COMPLEXES OF ANIMAL METAL-BINDING PROTEIN WITH PLATINUM CYTOSTATICS

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

The suggestion of interactions between heavy metals and biologic active molecules haven't been exactly estimated yet. However cadmium, lead or mercury are significant environment pollutants and platinum and arsenic are used as oncologic medicament, they have common characteristic. In organism these compounds are not volatile but they are bounded to other molecules. Interactions between heavy metals and proteins are important for range of physiologic processes like transpiration, photosynthesis or detoxification of organisms. In our experiment an electrochemical profile of interactions between 23 metallothionein fragments and cisplatin was studied. At first 23 MT fragments (decapeptides) were selected given by differences in aminoacids ordering. For the experiment amperometric detection implemented to flow injection analysis system (FIA-ED) was used. However a lot of results were estimated, we focused on complex formation between cisplatin and 23 MT fragments analyzed in various conditions. All the 23 MT fragments interacted with cisplatin, in the optional conditions as equimolar ratio, in physiological conditions of phosphate buffer (pH 7.5) in temperature of 37 degrees C. Based on results obtained we determined an interaction constant which defines an ability of each peptide to make an interaction with cisplatin. The highest IC was found by fragments 18 and 22 and the lowest IC by 1, 15, 12 and 19. We found that the major influence of interaction was done not by the change of near neighbouring aminoacids with the conservative cysteines byt these which were about 2 or 3 of positions far away from cysteiene.

Klíčová slova

Metallothionein; cisplatin; FIA; electrochemical detection

Autoři

ADAM, P.; ZÍTKA, O.; KOMÍNKOVÁ, M.; SKALIČKOVÁ, S.; SKUTKOVÁ, S.; ADAM, V.; KIZEK, R.

Vydáno

22. 11. 2012

ISBN

978-80-7375-836-3

Kniha

MENDELNET 2012

Strany od

1265

Strany do

1271

Strany počet

7

BibTex

@inproceedings{BUT145770,
  author="ADAM, P. and ZÍTKA, O. and KOMÍNKOVÁ, M. and SKALIČKOVÁ, S. and SKUTKOVÁ, S. and ADAM, V. and KIZEK, R.",
  title="STUDY OF COMPLEXES OF ANIMAL METAL-BINDING PROTEIN WITH PLATINUM CYTOSTATICS",
  booktitle="MENDELNET 2012",
  year="2012",
  pages="1265--1271",
  isbn="978-80-7375-836-3"
}