Detail publikace

Fibre coupled micro-light emitting diode array light source with integrated band-pass filter for fluorescence detection in miniaturised analytical systems

VACULOVIČOVÁ, M. AKTHER, M. MAASKANT, P. BRABAZON, D. MACKA, M.

Originální název

Fibre coupled micro-light emitting diode array light source with integrated band-pass filter for fluorescence detection in miniaturised analytical systems

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

In this work, a new type of miniaturized fibre-coupled solid-state light source is demonstrated as an excitation source for fluorescence detection in capillary electrophoresis. It is based on a parabolically shaped micro-light emitting diode (mu-LED) array with a custom band-pass optical interference filter (IF) deposited at the back of the LED substrate. The GaN mu-LED array consisted of 270 individual mu-LED elements with a peak emission at 470 nm, each about 14 mm in diameter and operated as a single unit. Light was extracted through the transparent substrate material, and coupled to an optical fibre (OF, 400 mm in diameter, numerical aperture NA = 0.37), to form an integrated mu-LED-IF-OF light source component. This packaged mu-LED-IF-OF light source emitted approximately 225 mu W of optical power at a bias current of 20 mA. The bandpass IF filter was designed to reduce undesirable LED light emissions in the wavelength range above 490 nm. Devices with and without IF were compared in terms of the optical power output, spectral characteristics as well as LOD values. While the IF consisted of only 7.5 pairs (15 layers) of SiO2/HfO2 layers, it resulted in an improvement of the baseline noise as well as the detection limit measured using fluorescein as test analyte, both by approximately one order of magnitude, with a LOD of 1 x 10(-8) mol L-1 obtained under optimised conditions. The mu-LED-IF-OF light source was then demonstrated for use in capillary electrophoresis with fluorimetric detection. The limits of detection obtained by this device were compared to those obtained with a commercial fibre coupled LED device.

Klíčová slova

Micro-light emitting diode array; Light source; Optical fibre; Capillary electrophoresis; Fluorescence detection; Separations

Autoři

VACULOVIČOVÁ, M.; AKTHER, M.; MAASKANT, P.; BRABAZON, D.; MACKA, M.

Vydáno

29. 4. 2015

ISSN

0003-2670

Periodikum

ANALYTICA CHIMICA ACTA

Ročník

871

Číslo

1

Stát

Nizozemsko

Strany od

85

Strany do

92

Strany počet

8

BibTex

@article{BUT145849,
  author="VACULOVIČOVÁ, M. and AKTHER, M. and MAASKANT, P. and BRABAZON, D. and MACKA, M.",
  title="Fibre coupled micro-light emitting diode array light source with integrated band-pass filter for fluorescence detection in miniaturised analytical systems",
  journal="ANALYTICA CHIMICA ACTA",
  year="2015",
  volume="871",
  number="1",
  pages="85--92",
  doi="10.1016/j.aca.2015.02.044",
  issn="0003-2670"
}