Detail publikace

Spectrophotometric Assay of Aflatoxin B1 Using Acetylcholinesterase Immobilized on Standard Microplates

POHANKA, M.

Originální název

Spectrophotometric Assay of Aflatoxin B1 Using Acetylcholinesterase Immobilized on Standard Microplates

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Aflatoxins are a group of hepatotoxic secondary metabolites produced by molds Aspergillus. During inappropriate storage or processing of food and beverages, aflatoxins can be distributed and become a serious health problem. Disparate analytical techniques were prepared for the assay in the past. Here, a method based on inhibition of enzyme acetylcholinesterase (AChE) is performed allowing fast and simple prove of aflatoxins presence. For the assay purposes, AChE was immobilized on standard polystyrene microplates using gelatin as a stabilizing substance. Both free and immobilized AChEs were used and compared one to each other. Immobilization protocol was optimized and interference of organic solvents such as methanol was tested. For the immobilized AChE, limit of detection 2.75 ppb for aflatoxin B1 was received. The immobilized AChE kept full activity at least one month. Suitability of the assay for a practical performance is considered.

Klíčová slova

Acetylcholinesterase; Aflatoxin; Aspergillus; Biosensor; Butyrylcholinesterase; Ellman method; Mycotoxin

Autoři

POHANKA, M.

Vydáno

24. 5. 2013

ISSN

0003-2719

Periodikum

ANALYTICAL LETTERS

Ročník

46

Číslo

8

Stát

Spojené státy americké

Strany od

1306

Strany do

1315

Strany počet

10

BibTex

@article{BUT145991,
  author="Miroslav {Pohanka}",
  title="Spectrophotometric Assay of Aflatoxin B1 Using Acetylcholinesterase Immobilized on Standard Microplates",
  journal="ANALYTICAL LETTERS",
  year="2013",
  volume="46",
  number="8",
  pages="1306--1315",
  doi="10.1080/00032719.2012.757703",
  issn="0003-2719"
}