Detail publikace

Rapid preparation of self-assembled CdTe quantum dots used for sensing of DNA in urine

NEJDL, L. ZELNÍČKOVÁ, J. VANĚČKOVÁ, T. HYNEK, D. ADAM, V. VACULOVIČOVÁ, M.

Originální název

Rapid preparation of self-assembled CdTe quantum dots used for sensing of DNA in urine

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

In this article, the authors report a systematic study of the self-assembly of CdTe quantum dots (QDs) stabilized by mercaptosuccinic acid (MSA) at laboratory temperature (25 degrees C) or after thermal treatment (90 degrees C). It was found that QD/MSA can be synthesized spontaneously under mild laboratory conditions (25 degrees C and 70 hours) without any further instrumentation or they can be treated using an increased temperature (90 degrees C and 8 min) to achieve the same fluorescence intensity. These QDs were used for the preparation of a fluorescent (off-on) probe based on methylene blue as a quencher for the specific determination of nucleic acid from urine. The whole process was monitored using stationary spectroscopy (absorbance and fluorescence measurement) and capillary electrophoresis (CE) with laser-induced fluorescence detection. The average size of the prepared QDs (approximately 3 nm) was determined using transmission electron microscopy and it was proven that the presented fluorescent probe combined with the CE method can be used for the sensitive determination of DNA (LOD 3 ng mL(-1)).

Klíčová slova

CdTe quantum dots; DNA in urine

Autoři

NEJDL, L.; ZELNÍČKOVÁ, J.; VANĚČKOVÁ, T.; HYNEK, D.; ADAM, V.; VACULOVIČOVÁ, M.

Vydáno

21. 4. 2018

ISSN

1144-0546

Periodikum

NEW JOURNAL OF CHEMISTRY

Ročník

42

Číslo

8

Stát

Spojené království Velké Británie a Severního Irska

Strany od

6005

Strany do

6012

Strany počet

8

BibTex

@article{BUT149400,
  author="Lukáš {Nejdl} and Jaroslava {Zelníčková} and Tereza {Vaněčková} and David {Hynek} and Vojtěch {Adam} and Markéta {Vaculovičová}",
  title="Rapid preparation of self-assembled CdTe quantum dots used for sensing of DNA in urine",
  journal="NEW JOURNAL OF CHEMISTRY",
  year="2018",
  volume="42",
  number="8",
  pages="6005--6012",
  doi="10.1039/c7nj05167k",
  issn="1144-0546"
}