Miniaturised and portable capillary liquid chromatography platforms with led photometric and electrochemical detection

abstrakt

angličtina

The impact of portable analytical technologies has been growing rapidly influenced by factors such as rising medical care costs and need for rapid and on-site answers. Liquid chromatography has developed over the last almost half a century into a powerful analytical technique recognised as essential in many areas for everyday analysis, however with limited advances allowing its use as a portable analytical technique. In pursuing miniaturised and portable LC, we have chosen a concept based on a modular LEGO-like system with miniaturised, mostly off-the-shelf components, assembled on a microfluidic breadboard, allowing great flexibility of design. The key components include miniaturised modular microsyringe pumps, electrically actuated microfluidic switching valves and a sample injection valve, a capillary column and UV-LED detector [1,2,3]. We investigated several UV LED-based photometric detection designs with focus on capillary z-cells which offer an order of magnitude higher sensitivity compared to on-capillary detection, but also minimal size and weight [4,5]. Further we pursued end-capillary electrochemical detection using to complement UV LED-based photometric detection and allow for amperometric detection of non-absorbing compounds. We investigated an end-capillary gap-flow cell (gap-FC) designed to use exchangeable screen-printed electrodes held in a miniature gap-FC [4]. This presentation outlines the concepts of portable LC instruments, explains the reasons behind our approach based on a miniaturised modular platform, details selected technical aspects focusing on detection, and discussed future developments.

Miniaturised and portable analysis; capillary liquid chromatography; LED photometric detection; electrochemical detection

MACKA, M.

25. 9. 2018

978-80-7560-168-1

Book of Abstracts INDC 2018

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