Přístupnostní navigace
E-přihláška
Vyhledávání Vyhledat Zavřít
Detail publikace
SMILKOVÁ, M. SMILEK, J. KALINA, M. KLUČÁKOVÁ, M. PEKAŘ, M. SEDLÁČEK, P.
Originální název
A simple technique for assessing the cuticular diffusion of humic acid biostimulants
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
Background: Experimental determination of the extent and rate of transport of liquid humates supplied to plants is critical in testing of physiological effects of such biostimulants which are often supplied as foliar sprays. Therefore, an original experimental method for the qualitative investigation and quantitative description of the penetration on humates through plant cuticles is proposed, tested, and evaluated. Results: The proposed method involves the isolation of model plant leaf cuticles and the subsequent in vitro evaluation of cuticular humate transport. The employed novel methodology is based on a simple diffusion couple arrangement involving continuous spectrophotometric determination of the amounts of penetrated humate in a hydrogel diffusion medium. Prunus laurocerasus leaf cuticles were isolated by chemical and enzymatic treatment and the rate of cuticular penetration of a commercial humate (lignohumate) was estimated over time in quantitative and qualitative terms. Different rates of lignohumate transport were determined for abaxial and adaxial leaf cuticles also in relation to the different cuticular extraction methods tested. Conclusions: The proposed methodology represents a simple and cheap experimental tool for the study on the trans-cuticular penetration of humic-based biostimulants.
Klíčová slova
Diffusion, Hydrogel, Prunus laurocerasus, Liquid fertilization, Humic substances, Plant cuticle
Autoři
SMILKOVÁ, M.; SMILEK, J.; KALINA, M.; KLUČÁKOVÁ, M.; PEKAŘ, M.; SEDLÁČEK, P.
Vydáno
31. 7. 2019
Nakladatel
BMC
ISSN
1746-4811
Periodikum
PLANT METHODS
Ročník
15
Číslo
1
Stát
Spojené království Velké Británie a Severního Irska
Strany od
Strany do
11
Strany počet
URL
https://plantmethods.biomedcentral.com/articles/10.1186/s13007-019-0469-x#Abs1
Plný text v Digitální knihovně
http://hdl.handle.net/11012/180811
BibTex
@article{BUT157782, author="Marcela {Smilková} and Jiří {Smilek} and Michal {Kalina} and Martina {Klučáková} and Miloslav {Pekař} and Petr {Sedláček}", title="A simple technique for assessing the cuticular diffusion of humic acid biostimulants", journal="PLANT METHODS", year="2019", volume="15", number="1", pages="1--11", doi="10.1186/s13007-019-0469-x", issn="1746-4811", url="https://plantmethods.biomedcentral.com/articles/10.1186/s13007-019-0469-x#Abs1" }