Detail publikace

Doxorubicin and ellipticine loaded apoferritin and its effect on cancer and healthy cell lines

ČERNÁ, T. HRABĚTA, J. INDRA, R. HEGER, Z. ADAM, V. ECKSCHLAGER, T. STIBOROVÁ, M.

Originální název

Doxorubicin and ellipticine loaded apoferritin and its effect on cancer and healthy cell lines

Typ

prezentace, poster

Jazyk

angličtina

Originální abstrakt

Despite advances in cancer diagnosis and therapy, high-risk neuroblastoma (NBL) remains lethal in about 50% of patients because of aggressive cell growth and chemoresistance. Therefore, new agents are needed to improve its prognosis. Apoferritin is a protein composed of 24 polypeptide subunits, structurally arranged to create an internal cavity, which can be artificially employed for carrying of some molecules of interest. The aim of this study was to compare the cytotoxic effects of doxorubicin (DOX) and ellipticine (ELLI) loaded apoferritin (APODOX resp. APOELLI) and free DOX resp. ELLI on NBL cells UKF-NB-4, chemoresistant sublines derived from this cell line and non-malignant fibroblasts (HDFn) in vitro. We show here that the effect of APODOX on NBL cells is similar to that of free DOX. The cytotoxicity of APOELLI is lower than cytotoxicity of free cytostatic, but APOELLI induces more double strand breaks than free ELLI. Further, using fluorescence microscopy, we have shown that apoferritin can deliver drugs inside cancer cells and the drug exerts their effect thereof. Entry of APODOX and free DOX into cancer cells was similar. However, entry of APOELLI was significantly lower than entry of free ELLI. The extent of APOELLI enter correlated with formation of covalent ELLIderived DNA adducts in tested cells; the levels of ELLI-DNA adducts generated by APOELLI were 67% of those formed by free ELLI. The results found in this study seem to be promising, because encapsulation does not affect toxicity of cytostatic and improves drug stability. Moreover, cytotoxicity of drug loaded apoferritin is significantly lower for non-malignant cells than for cancer cells. In addition, apoferritin loaded DOX and ELLI are cytotoxic in hypoxic conditions (1% O2). We suppose that apoferritin is targeted to the several cancer cells including NBL through TfR 1 and/or SCARA 5 which are expressed in many cancer cells.

Klíčová slova

Doxorubicin; apoferritin; cancer

Autoři

ČERNÁ, T.; HRABĚTA, J.; INDRA, R.; HEGER, Z.; ADAM, V.; ECKSCHLAGER, T.; STIBOROVÁ, M.

Vydáno

31. 7. 2018

Strany od

350

Strany do

350

Strany počet

1

URL

https://doi.org/10.1002/2211-5463.12453

BibTex

@misc{BUT163415,
  author="ČERNÁ, T. and HRABĚTA, J. and INDRA, R. and HEGER, Z. and ADAM, V. and ECKSCHLAGER, T. and STIBOROVÁ, M.",
  title="Doxorubicin and ellipticine loaded apoferritin and its effect on cancer and healthy cell lines
",
  year="2018",
  pages="350--350",
  url="https://doi.org/10.1002/2211-5463.12453",
  note="presentation, poster"
}