Detail publikace

UV-induced Zn:Cd/S quantum dots in-situ formed in the presence of thiols for sensitive and selective fluorescence detection of thiols

ASSI, N. NEJDL, L. ZEMÁNKOVÁ, K. PAVELICOVÁ, K. BEZDĚKOVÁ, J. MACKA, M. ADAM, V. VACULOVIČOVÁ, M.

Originální název

UV-induced Zn:Cd/S quantum dots in-situ formed in the presence of thiols for sensitive and selective fluorescence detection of thiols

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

In this work, we explored a new approach to a simple and sensitive fluorescence detection of thiols. The approach takes advantage of an in-situ formation of UV light-induced fluorescent nanoparticles (ZnCd/S quantum dots), while utilizing the thiol group of the analyte as a capping agent. The selectivity is ensured by the selective isolation of the thiol analyte by a polydopamine molecularly imprinted polymeric (MIP) layer. Based on this approach, a method for determination of thiols was designed. Key experimental parameters were optimized, including those of molecular imprinting and of effective model thiol molecule (l-cysteine) isolation. The relationship between the fluorescence intensity of ZnCd/S quantum dots and the concentration of l-cysteine in the range of 12-150 mu g/mL was linear with a detection limit of 3.6 mu g/mL. The molecularly imprinted polymer showed high absorption mass capacity (1.73 mg/g) and an excellent selectivity factor for l-cysteine compared to N-acetyl-l-cysteine and l-homocysteine of 63.56 and 87.48, respectively. The proposed method was applied for l-cysteine determination in human urine with satisfactory results. Due to a high variability of molecular imprinting technology and versatility of in-situ probe formation, methods based on this approach can be easily adopted for analysis of any thiol of interest.

Klíčová slova

thiol; molecularly imprinted polymer; polydopamine; urine

Autoři

ASSI, N.; NEJDL, L.; ZEMÁNKOVÁ, K.; PAVELICOVÁ, K.; BEZDĚKOVÁ, J.; MACKA, M.; ADAM, V.; VACULOVIČOVÁ, M.

Vydáno

5. 7. 2021

Nakladatel

Springer Nature

ISSN

2045-2322

Periodikum

Scientific Reports

Ročník

11

Číslo

1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

1

Strany do

11

Strany počet

11

URL

https://doi.org/10.1038/s41598-021-93137-4

Plný text v Digitální knihovně

http://hdl.handle.net/11012/200921

BibTex

@article{BUT172185,
  author="Navid {Assi} and Lukáš {Nejdl} and Kristýna {Zemánková} and Kristýna {Pavelicová} and Jaroslava {Bezděková} and Miroslav {Macka} and Vojtěch {Adam} and Markéta {Vaculovičová}",
  title="UV-induced Zn:Cd/S quantum dots in-situ formed in the presence of thiols for sensitive and selective fluorescence detection of thiols",
  journal="Scientific Reports",
  year="2021",
  volume="11",
  number="1",
  pages="1--11",
  doi="10.1038/s41598-021-93137-4",
  issn="2045-2322",
  url="https://doi.org/10.1038/s41598-021-93137-4"
}