Detail publikace

A Biological Study of Composites Based on the Blends of Nanohydroxyapatite, Silk Fibroin and Chitosan

Tuwalska, A. Sionkowska, A. Bryla, A. Tylko, G. Osyczka, AM. Laus, M. Vojtova, L.

Originální název

A Biological Study of Composites Based on the Blends of Nanohydroxyapatite, Silk Fibroin and Chitosan

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

In this work, the biological properties of three-dimensional scaffolds based on a blend of nanohydroxyapatite (nHA), silk fibroin (SF), and chitosan (CTS), were prepared using a lyophilization technique with various weight ratios: 10:45:45, 15:15:70, 15:70:15, 20:40:40, 40:30:30, and 70:15:15 nHA:SF:CTS, respectively. The basic 3D scaffolds were obtained from 5% (w/w) chitosan and 5% silk fibroin solutions and then nHA was added. The morphology and physicochemical properties of scaffolds were studied and compared. A biological test was performed to study the growth and osteogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs). It was found that the addition of chitosan increases the resistance properties and extends the degradation time of materials. In vitro studies with human mesenchymal stem cells found a high degree of biotolerance for the materials produced, especially for the 20:40:40 and 15:70:15 (nHa:SF:CTS) ratios. The presence of silk fibroin and the elongated shape of the pores positively influenced the differentiation of cells into osteogenic cells. By taking advantage of the differentiation/proliferation cues offered by individual components, the composites based on the nanohydroxyapatite, silk fibroin, and chitosan scaffold may be suitable for bone tissue engineering, and possibly offer an alternative to the widespread use of collagen materials.

Klíčová slova

silk fibroin; chitosan; hydroxyapatite; biological study; mesenchymal stem cells; bone regeneration; osteogenesis

Autoři

Tuwalska, A.; Sionkowska, A.; Bryla, A.; Tylko, G.; Osyczka, AM.; Laus, M.; Vojtova, L.

Vydáno

1. 8. 2022

Nakladatel

MDPI

Místo

BASEL

ISSN

1996-1944

Periodikum

Materials

Ročník

15

Číslo

15

Stát

Švýcarská konfederace

Strany od

5444

Strany do

5461

Strany počet

18

URL

https://www.mdpi.com/1996-1944/15/15/5444

BibTex

@article{BUT179221,
  author="Tuwalska, A. and Sionkowska, A. and Bryla, A. and Tylko, G. and Osyczka, AM. and Laus, M. and Vojtova, L.",
  title="A Biological Study of Composites Based on the Blends of Nanohydroxyapatite, Silk Fibroin and Chitosan",
  journal="Materials",
  year="2022",
  volume="15",
  number="15",
  pages="18",
  doi="10.3390/ma15155444",
  issn="1996-1944",
  url="https://www.mdpi.com/1996-1944/15/15/5444"
}