Detail publikace

New Multilocus Sequence Typing Scheme for Enterococcus faecium Based on Whole Genome Sequencing Data

BEZDÍČEK, M. HANSLIKOVA, J. NYKRÝNOVÁ, M. DUFKOVÁ, K. KOCMANOVÁ, I. KUBÁČKOVÁ, P. MAYER, J. LENGEROVÁ, M.

Originální název

New Multilocus Sequence Typing Scheme for Enterococcus faecium Based on Whole Genome Sequencing Data

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The MLST scheme currently used for Enterococcus faecium typing was designed in 2002 and is based on putative gene functions and Enterococcus faecalis gene sequences available at that time. As a result, the original MLST scheme does not correspond to the real genetic relatedness of E. faecium strains and often clusters genetically distant strains to the same sequence types (ST). Nevertheless, typing has a significant impact on the subsequent epidemiological conclusions and introduction of appropriate epidemiological measures, thus it is crucial to use a more accurate MLST scheme. Based on the genome analysis of 1,843 E. faecium isolates, a new scheme, consisting of 8 highly discriminative loci, was created in this study. These strains were divided into 421 STs using the new MLST scheme, as opposed to 223 STs assigned by the original MLST scheme. The proposed MLST has a discriminatory power of D = 0.983 (CI95% 0.981 to 0.984), compared to the original scheme’s D = 0.919 (CI95% 0.911 to 0.927). Moreover, we identified new clonal complexes with our newly designed MLST scheme. The scheme proposed here is available within the PubMLST database. Although whole genome sequencing availability has increased rapidly, MLST remains an integral part of clinical epidemiology, mainly due to its high standardization and excellent robustness. In this study, we proposed and validated a new MLST scheme for E. faecium, which is based on genome-wide data and thus reflects the tested isolates’ more accurate genetic similarity.

Klíčová slova

Enterococcus faesium; multilocus sequence typing; clonal complex; epidemiology; whole genome sequenging

Autoři

BEZDÍČEK, M.; HANSLIKOVA, J.; NYKRÝNOVÁ, M.; DUFKOVÁ, K.; KOCMANOVÁ, I.; KUBÁČKOVÁ, P.; MAYER, J.; LENGEROVÁ, M.

Vydáno

12. 6. 2023

Nakladatel

American Society for Microbiology

ISSN

2165-0497

Periodikum

Microbiology spectrum

Ročník

11

Číslo

4

Stát

Spojené státy americké

Strany od

1

Strany do

9

Strany počet

9

URL

https://journals.asm.org/doi/10.1128/spectrum.05107-22

Plný text v Digitální knihovně

http://hdl.handle.net/11012/244971

BibTex

@article{BUT183746,
  author="Matěj {Bezdíček} and Jana {Hanslikova} and Markéta {Jakubíčková} and Kristýna {Dufková} and Iva {Kocmanová} and Petra {Kubáčková} and Jiří {Mayer} and Martina {Lengerová}",
  title="New Multilocus Sequence Typing Scheme for Enterococcus faecium Based on Whole Genome Sequencing Data",
  journal="Microbiology spectrum",
  year="2023",
  volume="11",
  number="4",
  pages="1--9",
  doi="10.1128/spectrum.05107-22",
  issn="2165-0497",
  url="https://journals.asm.org/doi/10.1128/spectrum.05107-22"
}