Detail publikace

Homogeneous films from amphiphilic hyaluronan and their characterization by confocal microscopy and nanoindentation

MRÁZEK, J. SITA, J. ONDREÁŠ, F. ZUBOV, A. CHMELAŘ, J.

Originální název

Homogeneous films from amphiphilic hyaluronan and their characterization by confocal microscopy and nanoindentation

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Self-supporting films from amphiphilic hyaluronan are suitable for medical applications like wound dressings or resorbable implants. These films are typically cast from water/alcohol solutions. However, when the mixed solvent evaporates in ambient air, convection flows develop in the solution and become imprinted in the film, potentially compromising its properties. Consequently, we developed a novel film manufacturing method: drying in a closed box under saturated vapour conditions. Using this approach, we prepared a series of optically clear lauroyl-hyaluronan (LHA) films with uniform thickness and compared them to their air-dried counterparts. We first evaluated swelling ratios and elastic moduli for LHA films with varying degrees of substitution. The boxdried films swelled significantly less and were 1-2 orders of magnitude stiffer than air-dried films from the same LHA sample. Confocal microscopy revealed that box-dried films exhibited a regular microstructure, while air-dried films displayed a pore-size gradient and strong microstructure modulation due to convection flows. Local elastic modulus variations arising from these microstructures were assessed using nanoindentation mapping. Importantly, achieving the desired film stiffness requires much lower polymer modification when boxdrying is used, enhancing the biological response to the material. These findings have implications for all polysaccharide formulations that utilize mixed solvents.

Klíčová slova

Hyaluronan; Film; Hydrogel; Solution casting; Mixed solvent; Microstructure; Nanoindentation

Autoři

MRÁZEK, J.; SITA, J.; ONDREÁŠ, F.; ZUBOV, A.; CHMELAŘ, J.

Vydáno

27. 5. 2024

Nakladatel

ELSEVIER SCI LTD

Místo

London

ISSN

0144-8617

Periodikum

Carbohydrate Polymers

Ročník

340

Číslo

122331

Stát

Spojené království Velké Británie a Severního Irska

Strany počet

10

URL

https://www.sciencedirect.com/science/article/pii/S0144861724005575?via=ihub

BibTex

@article{BUT189030,
  author="MRÁZEK, J. and SITA, J. and ONDREÁŠ, F. and ZUBOV, A. and CHMELAŘ, J.",
  title="Homogeneous films from amphiphilic hyaluronan and their characterization by confocal microscopy and nanoindentation",
  journal="Carbohydrate Polymers",
  year="2024",
  volume="340",
  number="122331",
  pages="10",
  doi="10.1016/j.carbpol.2024.122331",
  issn="0144-8617",
  url="https://www.sciencedirect.com/science/article/pii/S0144861724005575?via=ihub"
}