Detail publikačního výsledku

Exploring DNA Methylation Patterns in the Core Genome of Klebsiella pneumoniae

NYKRÝNOVÁ, M.; BEZDÍČEK, M.; LENGEROVÁ, M.; VÍTKOVÁ, H.

Originální název

Exploring DNA Methylation Patterns in the Core Genome of Klebsiella pneumoniae

Anglický název

Exploring DNA Methylation Patterns in the Core Genome of Klebsiella pneumoniae

Druh

Stať ve sborníku v databázi WoS či Scopus

Originální abstrakt

In recent years, sequencing has become easily accessible and widespread, revolutionizing our ability to study complex genetic information. Thus, it is possible to analyze complex bacterial populations and investigate relationships between individual strains. Methods such as core genome multilocus sequence typing (cgMLST) are used in routine clinical practice to characterize bacterial isolates, track the spread of infectious diseases, and monitor outbreaks. However, the limitation lies in distinguishing closely related bacterial strains, which differ only in several bases. Therefore, here, we present the core genome methylome analysis utilizing nanopore sequencing. We demonstrate that epigenetic information contained within bacterial strains allows us to differentiate populations similarly to cgMLST. Moreover, the proposed unique combination of cgMLST with core genome methylome can even increase the discriminatory power between closely similar isolates, overcoming the constraints of cgMLST. Combining genomic and epigenomic information can provide better insight into bacterial strains’ evolution, transmission patterns and pathogenicity study.

Anglický abstrakt

In recent years, sequencing has become easily accessible and widespread, revolutionizing our ability to study complex genetic information. Thus, it is possible to analyze complex bacterial populations and investigate relationships between individual strains. Methods such as core genome multilocus sequence typing (cgMLST) are used in routine clinical practice to characterize bacterial isolates, track the spread of infectious diseases, and monitor outbreaks. However, the limitation lies in distinguishing closely related bacterial strains, which differ only in several bases. Therefore, here, we present the core genome methylome analysis utilizing nanopore sequencing. We demonstrate that epigenetic information contained within bacterial strains allows us to differentiate populations similarly to cgMLST. Moreover, the proposed unique combination of cgMLST with core genome methylome can even increase the discriminatory power between closely similar isolates, overcoming the constraints of cgMLST. Combining genomic and epigenomic information can provide better insight into bacterial strains’ evolution, transmission patterns and pathogenicity study.

Klíčová slova

methylome; cgMLST; bacteria; resistance

Klíčová slova v angličtině

methylome; cgMLST; bacteria; resistance

Autoři

NYKRÝNOVÁ, M.; BEZDÍČEK, M.; LENGEROVÁ, M.; VÍTKOVÁ, H.

Rok RIV

2025

Vydáno

28.08.2024

Nakladatel

Springer Nature

ISBN

978-3-031-64636-2

Kniha

Lecture Notes in Bioinformatics

Edice

14849

ISSN

1611-3349

Periodikum

Lecture Notes in Computer Science

Svazek

14849

Číslo

II.

Stát

Švýcarská konfederace

Strany od

140

Strany do

152

Strany počet

13

URL

https://link.springer.com/chapter/10.1007/978-3-031-64636-2_11

BibTex

@inproceedings{BUT189432,
  author="Markéta {Jakubíčková} and Matěj {Bezdíček} and Martina {Lengerová} and Helena {Vítková}",
  title="Exploring DNA Methylation Patterns in the Core Genome of Klebsiella pneumoniae",
  booktitle="Lecture Notes in Bioinformatics",
  year="2024",
  series="14849",
  journal="Lecture Notes in Computer Science",
  volume="14849",
  number="II.",
  pages="140--152",
  publisher="Springer Nature",
  doi="10.1007/978-3-031-64636-2\{_}11",
  isbn="978-3-031-64636-2",
  url="https://link.springer.com/chapter/10.1007/978-3-031-64636-2_11"
}