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OMELKOVÁ, J.
Originální název
Purification and biochemical characterization of polygalacturonases produced by Aureobasidium pullulans
Typ
článek v časopise - ostatní, Jost
Jazyk
angličtina
Originální abstrakt
The extracellular polygalacturonases produced by Aureobasidium pullulans isolated from waters of the Danube river were partially purified and characterized. The pH optima of polygalacturonases produced in the first phases of cultivation (48 h) and after 10 d as well as their optima of temperature, thermal stabilities, molecular masses, isoelectric points, action pattern and ability to cleave polymeric and oligomeric substrates were compared. Polygalacturonases with a random action pattern (random cleavage of pectate forming a mixture of galactosiduronides with a lower degree of polymerization) [EC 3.2.1.15] were produced only in the first phases of growth, while exopolygalacturonases [EC 3.2.1.67] with a terminal action pattern (cleavage of pectate from the nonreducing end forming D-galactopyranuronic acid as a product) were found during the whole growth. The main enzyme form with a random action pattern was glycosylated and its active site had the arrangement described previously for the active site of polygalacturonase of phytopathogenic fungi.
Klíčová slova
Aureobasidium pullulans; exopolygalacturonase; polygalacturonase
Autoři
Rok RIV
2005
Vydáno
31. 1. 2005
Nakladatel
VERLAG Z NATURFORSCH
ISSN
0939-5075
Periodikum
ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES
Ročník
2005 (3)
Číslo
60
Stát
Spolková republika Německo
Strany od
91
Strany do
96
Strany počet
7
BibTex
@article{BUT46093, author="Jiřina {Omelková}", title="Purification and biochemical characterization of polygalacturonases produced by Aureobasidium pullulans", journal="ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES", year="2005", volume="2005 (3)", number="60", pages="91--96", issn="0939-5075" }