Detail publikace

Phytochelatin synthase activity as a marker of metal pollution

ZÍTKA, O. KRYŠTOFOVÁ, O. ŠOBROVÁ, P. ADAM, V. ZEHNÁLEK, J. BEKLOVÁ, M. KIZEK, R.

Originální název

Phytochelatin synthase activity as a marker of metal pollution

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The synthesis of phytochelatins is catalyzed by gamma-Glu-Cys dipeptidyl transpeptidase called phytochelatin synthase (PCS). Aim of this study was to suggest a new tool for determination of phytochelatin synthase activity in the tobacco BY-2 cells treated with different concentrations of the Cd(II). After the optimization steps, an experiment on BY-2 cells exposed to different concentrations of Cd(NO3)2 for 3 days was performed. At the end of the experiment, cells were harvested and homogenized. Reduced glutathione and cadmium (II) ions were added to the cell suspension supernatant. These mixtures were incubated at 35 C for 30 min and analysed using high performance liquid chromatography coupled with electrochemical detector (HPLC-ED). The results revealed that PCS activity rises markedly with increasing concentration of cadmium (II) ions. The lowest concentration of the toxic metal ions caused almost three fold increase in PCS activity as compared to control samples. The activity of PCS (270 fkat) in treated cells was more than seven times higher in comparison to control ones. Km for PCS was estimated as 2.3 mM.

Klíčová slova

Phytochelatin synthase activity, Cadmium, Glutathione, High performance liquid chromatography with electrochemical detection, Tobacco BY-2 cell suspension

Autoři

ZÍTKA, O.; KRYŠTOFOVÁ, O.; ŠOBROVÁ, P.; ADAM, V.; ZEHNÁLEK, J.; BEKLOVÁ, M.; KIZEK, R.

Rok RIV

2011

Vydáno

2. 6. 2011

Nakladatel

Elsevier

ISSN

0304-3894

Periodikum

Journal of Hazardous Materials

Ročník

2011 (192)

Číslo

1

Stát

Nizozemsko

Strany od

794

Strany do

800

Strany počet

7

BibTex

@article{BUT75096,
  author="Ondřej {Zítka} and Olga {Kryštofová} and Pavlína {Adam} and Vojtěch {Adam} and Josef {Zehnálek} and Miroslava {Beklová} and René {Kizek}",
  title="Phytochelatin synthase activity as a marker of metal pollution",
  journal="Journal of Hazardous Materials",
  year="2011",
  volume="2011 (192)",
  number="1",
  pages="794--800",
  doi="10.1016/j.jhazmat.2011.05.088",
  issn="0304-3894"
}