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DRBOHLAVOVÁ, J. CHOMOUCKÁ, J. HRDÝ, R. PRÁŠEK, J. JANŮ, L. RYVOLOVÁ, M. ADAM, V. KIZEK, R. HALASOVÁ, T. HUBÁLEK, J.
Originální název
Effect of Nucleic Acid and Albumin on Luminescence Properties of Deposited TiO2 Quantum Dots
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
Luminescent quantum dots (QDs) have gained a great attention as promising candidates in optical detection of various biomolecules such as DNA, proteins, etc. In this paper, we focused on fabrication of QDs in the deposited form, which still belongs to less clarified and used approach in biosensing compared to colloidal one. The deposited QDs from non-toxic titanium dioxide (TiO2) were prepared by template based technique, namely anodic oxidation of titanium film through nanoporous alumina template. This method allows to creation of QDs strongly fixed on silicon substrate without addition of any other fixation matrix (e.g. polymer). Highly ordered TiO2 QDs with broad luminescence peak in visible range were achieved. The QDs array was characterized by SEM confirming the QDs size being below 10 nm, which is necessary to reach the quantum size effect. Preliminary results showed that subsequent modification of QDs surface with chicken DNA and bovine serum albumin (BSA) resulted in partial or complete quenching of fluorescence depending on the biomolecules concentration.
Klíčová slova
quantum dots; luminescence; biosensors; TiO2; template method; DNA; bovine serum albumin
Autoři
DRBOHLAVOVÁ, J.; CHOMOUCKÁ, J.; HRDÝ, R.; PRÁŠEK, J.; JANŮ, L.; RYVOLOVÁ, M.; ADAM, V.; KIZEK, R.; HALASOVÁ, T.; HUBÁLEK, J.
Rok RIV
2012
Vydáno
1. 2. 2012
Nakladatel
ESG
Místo
Bělehrad, Srbsko
ISSN
1452-3981
Periodikum
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
Ročník
7
Číslo
2
Stát
Srbská republika
Strany od
1424
Strany do
1432
Strany počet
9
BibTex
@article{BUT75689, author="DRBOHLAVOVÁ, J. and CHOMOUCKÁ, J. and HRDÝ, R. and PRÁŠEK, J. and JANŮ, L. and RYVOLOVÁ, M. and ADAM, V. and KIZEK, R. and HALASOVÁ, T. and HUBÁLEK, J.", title="Effect of Nucleic Acid and Albumin on Luminescence Properties of Deposited TiO2 Quantum Dots", journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE", year="2012", volume="7", number="2", pages="1424--1432", issn="1452-3981" }