Detail publikace

Cadmium(II) and zinc(II) ions effects on maize plants revealed by spectroscopy and electrochemistry

KLECKEROVÁ, A. SOBROVÁ, P. KRYŠTOFOVÁ, O. SOCHOR, J. ZÍTKA, O. BABULA, P. ADAM, V. DOČEKALOVÁ, H. KIZEK, R.

Originální název

Cadmium(II) and zinc(II) ions effects on maize plants revealed by spectroscopy and electrochemistry

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Toxicity of cadmium has been described in numerous papers and includes especially ability to generate reactive oxygen species (ROS), which are responsible for damaging of biomolecules and loss of their biological functions, however, mutual effect of zinc(II) and cadmium(II) ions is still unclear. Growth parameters (root/shoot length, fresh and dry weight), and stress markers and enzymes (oxidized/reduced glutathione, phytochelatins, total content of thiols, activities of glutathione-S-transferase (GST), alanine transaminase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP)) were monitored during the treatment. In addition, total content of zinc(II) and cadmium(II) ions was determined in plant tissues electrochemically using differential pulse anodic stripping voltammetry. Moreover, content of reduced (GSH) and oxidized (GSSG) glutathione, and phytochelatin2 and phytochelatin5 were quantified using high performance liquid chromatography with electrochemical detection (HPLC-ED). Firstly, we studied the influence of metal ions on the growth parameters, i.e. fresh/dry weight and root/shoot length. Application of both metals led to the reduction of root as well as shoot biomass compared to control untreated plants. These adverse effects were found also in length of shoot and roots as well as dry weight. Content of both cadmium and zinc was determined in roots and shoots of experimental plants using Differential Pulse Anodic Stripping Voltammetry (DPASV). Amount of thiol compounds as well as GST activity was two times higher in roots compared to shoots. Aiming our attention on content of particular thiols, significant increasing of total content of GSH was observed at both 2nd and 10th day of the treatment. The most significant changes were evident in the case of the highest joint concentrations of zinc(II) and cadmium(II) ions - 100 uM Zn2+ + 100 uM Cd2+. Treatment with both cadmium(II) and/or zinc(II) ions led to the increase of PC2 and PC5 in experimental plants. Higher amount of phytochelatins in shoots compared to roots indicates their role in chelatation and transport of both heavy metals to aerial parts.

Klíčová slova

stress markers, glutathione-S-transferase, alanine transaminase, aspartate transaminase, alkaline phosphatase, Differential Pulse Anodic Stripping Voltammetry

Autoři

KLECKEROVÁ, A.; SOBROVÁ, P.; KRYŠTOFOVÁ, O.; SOCHOR, J.; ZÍTKA, O.; BABULA, P.; ADAM, V.; DOČEKALOVÁ, H.; KIZEK, R.

Rok RIV

2011

Vydáno

1. 12. 2011

ISSN

1452-3981

Periodikum

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

Ročník

2011 (6)

Číslo

12

Stát

Srbská republika

Strany od

6011

Strany do

6031

Strany počet

21

BibTex

@article{BUT75729,
  author="Andrea {Ridošková} and Pavlína {Adam} and Olga {Kryštofová} and Jiří {Sochor} and Ondřej {Zítka} and Petr {Babula} and Vojtěch {Adam} and Hana {Dočekalová} and René {Kizek}",
  title="Cadmium(II) and zinc(II) ions effects on maize plants revealed by spectroscopy and electrochemistry",
  journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE",
  year="2011",
  volume="2011 (6)",
  number="12",
  pages="6011--6031",
  issn="1452-3981"
}