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SVOBODA, O. FOHLEROVÁ, Z. BAIAZITOVA, L. ČMIEL, V. HUBÁLEK, J. PROVAZNÍK, I. PEKAŘÍK, V.
Originální název
MATRA-A reagent/polyethyleneimine complex transfection for low toxic DNA plasmid delivery
Typ
článek ve sborníku mimo WoS a Scopus
Jazyk
angličtina
Originální abstrakt
The non-toxic intracellular delivery of biopolymers is very hot topic in current biology. In this work, two different MATRA-A reagent/polyethyleneimine transfection protocols were used for intracellular delivery of ChR2 DNA vector. Cell morphology changes were studied 48 hours after transfection. Our transfection efficiency is lower than normally achieved, but the rate of cells with physiological properties remains is very high: 85.34%. Therefore, these transfection protocols are very applicable for electrophysiological experiments.
Klíčová slova
transfection, MATRA-A reagent, plasmid delivery
Autoři
SVOBODA, O.; FOHLEROVÁ, Z.; BAIAZITOVA, L.; ČMIEL, V.; HUBÁLEK, J.; PROVAZNÍK, I.; PEKAŘÍK, V.
Vydáno
6. 5. 2016
Nakladatel
Masarykova Univerzita - MUNI PRESS
Místo
Brno, Czech Republic
ISBN
978-80-210-8267-0
Kniha
XVI. Workshop of Physical Chemists and Electrochemists
Edice
1
Strany od
Strany do
3
Strany počet
BibTex
@inproceedings{BUT128268, author="Ondřej {Svoboda} and Zdenka {Fohlerová} and Larisa {Chmelíková} and Vratislav {Čmiel} and Jaromír {Hubálek} and Valentine {Provazník} and Vladimír {Pekařík}", title="MATRA-A reagent/polyethyleneimine complex transfection for low toxic DNA plasmid delivery", booktitle="XVI. Workshop of Physical Chemists and Electrochemists", year="2016", series="1", pages="1--3", publisher="Masarykova Univerzita - MUNI PRESS", address="Brno, Czech Republic", isbn="978-80-210-8267-0" }