Přístupnostní navigace
E-přihláška
Vyhledávání Vyhledat Zavřít
Detail publikace
SLAVÍK, J. ČMIEL, V. HUBÁLEK, J. YANG, Y. REN, T.-L.
Originální název
Hippocampal Neurons’ Alignment on Quartz Grooves and Parylene Cues on Quartz Substrate
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
Alignment and patterning of neurons have great importance in some research fields, especially for regenerative medicine and for the formation of artificial neural networks. Alignment of neurons on quartz grooves and parylene cues on quartz substrate was evaluated in this work. The neurons’ alignment on quartz grooves is considered to be topographical alignment, while the neurons’ alignment on parylene cues on quartz substrate is considered to be chemical alignment. Both quartz grooves’ and parylene cues’ widths were fabricated in a range from 2 µm to 8 µm; quartz grooves’ heights were in a range from 0.25 µm to 4 µm, while parylene cues’ heights were only 0.25 µm. Neurons were dissociated hippocampal neurons from rat E18. Neurons were cultivated on test substrates for 7 days before alignment evaluation. As expected, neurons aligned according to the direction of grooves and cues; however, transversal growth direction was also observed with much smaller tendency. Chemical alignment was found to be more effective than topographical alignment. If parylene cues are thin and distanced enough, then neurons have a tendency to follow the direction of individual parylene cues; however, neurons on quartz grooves have a tendency just to follow a preferable direction than individual quartz grooves.
Klíčová slova
neurons, quartz grooves, parylene cues, neurons alighment, neurons analysis
Autoři
SLAVÍK, J.; ČMIEL, V.; HUBÁLEK, J.; YANG, Y.; REN, T.-L.
Vydáno
30. 12. 2020
Nakladatel
MDPI AG
ISSN
2076-3417
Periodikum
Applied Sciences - Basel
Ročník
11
Číslo
1
Stát
Švýcarská konfederace
Strany od
Strany do
12
Strany počet
URL
https://www.mdpi.com/2076-3417/11/1/275
Plný text v Digitální knihovně
http://hdl.handle.net/11012/195846
BibTex
@article{BUT167392, author="Jan {Slavík} and Vratislav {Čmiel} and Jaromír {Hubálek} and Yi {Yang} and Tian-Ling {Ren}", title="Hippocampal Neurons’ Alignment on Quartz Grooves and Parylene Cues on Quartz Substrate", journal="Applied Sciences - Basel", year="2020", volume="11", number="1", pages="1--12", doi="10.3390/app11010275", issn="2076-3417", url="https://www.mdpi.com/2076-3417/11/1/275" }