Detail publikace

Computational image enhancement of multimode fibre-based holographic endo-microscopy: harnessing the muddy modes

TUČKOVÁ, T. ŠILER, M. FLAES, D. JÁKL, P. TURTAEV, S. KRÁTKÝ, S. HEINTZMANN, R. UHLÍŘOVÁ, H. ČIŽMÁR, T.

Originální název

Computational image enhancement of multimode fibre-based holographic endo-microscopy: harnessing the muddy modes

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

In imaging geometries, which employ wavefront-shaping to control the light transport through a multi-mode optical fibre (MMF), this terminal hair-thin optical component acts as a minimally invasive objective lens, enabling high resolution laser-scanning fluorescence microscopy inside living tissues at depths hardly accessible by any other light-based technique. Even in the most advanced systems, the diffraction-limited foci scanning the object across the focal plane are contaminated by a stray optical signal carrying typically few tens of % of the total optical power. The stray illumination takes the shape of a randomised but reproducible speckle, and is unique for each position of the focus. We experimentally demonstrate that the performance of imaging a fluorescent object can be significantly improved, when resulting images are computationally post-processed, utilising records of intensities of all speckle-contaminated foci used in the imaging procedure. We present two algorithms based on a regularised iterative inversion and regularised direct pseudo-inversion respectively which lead to enhancement of the image contrast and resolution. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

Klíčová slova

LIGHT; TRANSFORMATION; TRANSMISSION; RESOLUTION; PHASE

Autoři

TUČKOVÁ, T.; ŠILER, M.; FLAES, D.; JÁKL, P.; TURTAEV, S.; KRÁTKÝ, S.; HEINTZMANN, R.; UHLÍŘOVÁ, H.; ČIŽMÁR, T.

Vydáno

8. 11. 2021

Nakladatel

Optica Publishing Group

Místo

WASHINGTON

ISSN

1094-4087

Periodikum

OPTICS EXPRESS

Ročník

29

Číslo

23

Stát

Spojené státy americké

Strany od

38206

Strany do

38220

Strany počet

15

URL

https://www.osapublishing.org/oe/fulltext.cfm?uri=oe-29-23-38206&id=464465

Plný text v Digitální knihovně

http://hdl.handle.net/11012/203100

BibTex

@article{BUT174094,
  author="Tereza {Chmelíková} and Martin {Šiler} and Dirk E. Boonzajer {Flaes} and Petr {Jákl} and Sergey {Turtaev} and Stanislav {Krátký} and Rainer {Heintzmann} and Hana {Uhlířová} and Tomáš {Čižmár}",
  title="Computational image enhancement of multimode fibre-based holographic endo-microscopy: harnessing the muddy modes",
  journal="OPTICS EXPRESS",
  year="2021",
  volume="29",
  number="23",
  pages="38206--38220",
  doi="10.1364/OE.434848",
  issn="1094-4087",
  url="https://www.osapublishing.org/oe/fulltext.cfm?uri=oe-29-23-38206&id=464465"
}