Detail publikace

Genetic engineering of low-temperature polyhydroxyalkanoate production by Acidovorax sp. A1169, a psychrophile isolated from a subglacial outflow

GRZESIAK, J. GAWOR, J. ROGALA, M. KOUŘILOVÁ, X. OBRUČA, S.

Originální název

Genetic engineering of low-temperature polyhydroxyalkanoate production by Acidovorax sp. A1169, a psychrophile isolated from a subglacial outflow

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

In recent years, extremophilic microorganisms have been employed as producers of the microbial bioplastics polyhydroxyalkanoates (PHA), which are of great biotechnological value. Nevertheless, cold-loving or psychrophilic (cryophilic) bacteria have been neglected in this regard. Here, we present an investigation of the Arctic glacier-derived PHA producer Acidovorax sp. A1169. Biolog GEN III Microplates were used as a screening tool to identify the most suitable carbon substrate concerning PHA synthesis. The strain produced homopolymer poly(3-hydroxybutyrate) (PHB) most efficiently (2 g/L) at a temperature of 15 ? when supplied with fructose or mannitol as carbon sources with a substantial decrease of PHB biosynthesis at 17.5 ?. The PHB yield did not increase considerably or even decreased when carbon source concentration exceeded 10 g/L hinting that the strain is oligotrophic in nature. The strain was also capable of introducing 3-hydroxyvalerate (3HV) into the polymer structure, which is known to improve PHA thermoplastic properties. This is the first investigation providing insight into a PHA biosynthesis process by means of a true psychrophile, offering guidelines on polar-region bacteria cultivation, production of PHA and also on the methodology for genetic engineering of psychrophiles.

Klíčová slova

Extremophile; Arctic; Bioplastics; Low-temperature; biotechnology; Oligotrophy

Autoři

GRZESIAK, J.; GAWOR, J.; ROGALA, M.; KOUŘILOVÁ, X.; OBRUČA, S.

Vydáno

1. 12. 2023

Nakladatel

Springer

ISSN

1431-0651

Periodikum

EXTREMOPHILES

Ročník

27

Číslo

3

Stát

Japonsko

Strany od

1

Strany do

14

Strany počet

14

URL

https://link.springer.com/article/10.1007/s00792-023-01311-5

Plný text v Digitální knihovně

http://hdl.handle.net/11012/245178

BibTex

@article{BUT184904,
  author="Jakub {Grzesiak} and Jan {Gawor} and Małgorzata Marta {Rogala} and Xenie {Kouřilová} and Stanislav {Obruča}",
  title="Genetic engineering of low-temperature polyhydroxyalkanoate production by Acidovorax sp. A1169, a psychrophile isolated from a subglacial outflow",
  journal="EXTREMOPHILES",
  year="2023",
  volume="27",
  number="3",
  pages="1--14",
  doi="10.1007/s00792-023-01311-5",
  issn="1431-0651",
  url="https://link.springer.com/article/10.1007/s00792-023-01311-5"
}