Detail publikace

Static 3D Osteoblast Cell Culture on 3D Printed Titanium Scaffolds

OLIVER URRUTIA, C. DIEZ-ESCUDERO, A. KOLEDOVÁ, Z. ČELKO, L. HAILER, N. P. MONTUFAR JIMENEZ, E.

Originální název

Static 3D Osteoblast Cell Culture on 3D Printed Titanium Scaffolds

Typ

kapitola v knize

Jazyk

angličtina

Originální abstrakt

In vitro cell cultures are a very useful tool for the validation of biomaterial cytocompatibility, especially for bone tissue engineering scaffolds and bone implants. In this chapter, a protocol for a static three-dimensional osteoblast cell culture on titanium scaffolds and subsequent analysis of osteogenic capacity is presented. The protocol is explained for additively manufactured titanium scaffolds, but it can be extrapolated to other scaffolds with similar size and structure, while differing in composition or manufactured technology. Additionally, the protocol can be used for culture of other adherent cell types beyond osteoblast cells such as mesenchymal stem cells.

Klíčová slova

cell cultures; In vitro; cytocompatibility; titanium scaffolds

Autoři

OLIVER URRUTIA, C.; DIEZ-ESCUDERO, A.; KOLEDOVÁ, Z.; ČELKO, L.; HAILER, N. P.; MONTUFAR JIMENEZ, E.

Vydáno

24. 2. 2024

Nakladatel

Humana

Místo

New York, NY

ISBN

978-1-0716-3673-2

Kniha

Methods in Molecular Biology

Edice

2764

Strany od

43

Strany do

60

Strany počet

17

URL

https://link.springer.com/protocol/10.1007/978-1-0716-3674-9_5#citeas

BibTex

@inbook{BUT188701,
  author="OLIVER URRUTIA, C. and DIEZ-ESCUDERO, A. and KOLEDOVÁ, Z. and ČELKO, L. and HAILER, N. P. and MONTUFAR JIMENEZ, E.",
  title="Static 3D Osteoblast Cell Culture on 3D Printed Titanium Scaffolds",
  booktitle="Methods in Molecular Biology",
  year="2024",
  publisher="Humana",
  address="New York, NY",
  series="2764",
  pages="43--60",
  doi="10.1007/978-1-0716-3674-9\{_}5",
  isbn="978-1-0716-3673-2",
  url="https://link.springer.com/protocol/10.1007/978-1-0716-3674-9_5#citeas"
}