Detail publikace

Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment

KROUPOVÁ, Z. SLANINOVÁ, E. MRÁZOVÁ, K. KRZYŽÁNEK, V. HRUBANOVÁ, K. FRITZ, I. OBRUČA, S.

Originální název

Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Cyanobacteria are prokaryotic organisms characterised by their complex structures and a wide range of pigments. With their ability to fix CO2, cyanobacteria are interesting for white biotechnology as cell factories to produce various high-value metabolites such as polyhydroxyalkanoates, pigments, or proteins. White biotechnology is the industrial production and processing of chemicals, materials, and energy using microorganisms. It is known that exposing cyanobacteria to low levels of stressors can induce the production of secondary metabolites. Understanding of this phenomenon, known as hormesis, can involve the strategic application of controlled stressors to enhance the production of specific metabolites. Consequently, precise measurement of cyanobacterial viability becomes crucial for process control. However, there is no established reliable and quick viability assay protocol for cyanobacteria since the task is challenging due to strong interferences of autofluorescence signals of intercellular pigments and fluorescent viability probes when flow cytometry is used. We performed the screening of selected fluorescent viability probes used frequently in bacteria viability assays. The results of our investigation demonstrated the efficacy and reliability of three widely utilised types of viability probes for the assessment of the viability of Synechocystis strains. The developed technique can be possibly utilised for the evaluation of the importance of polyhydroxyalkanoates for cyanobacterial cultures with respect to selected stressor-repeated freezing and thawing. The results indicated that the presence of polyhydroxyalkanoate granules in cyanobacterial cells could hypothetically contribute to the survival of repeated freezing and thawing.

Klíčová slova

Biotechnology; Cyanobacteria; Flow cytometry; Fluorescent viability probes; Stress resistance; Viability assessment

Autoři

KROUPOVÁ, Z.; SLANINOVÁ, E.; MRÁZOVÁ, K.; KRZYŽÁNEK, V.; HRUBANOVÁ, K.; FRITZ, I.; OBRUČA, S.

Vydáno

6. 11. 2024

Nakladatel

SPRINGER

Místo

DORDRECHT

ISSN

1874-9356

Periodikum

FOLIA MICROBIOLOGICA

Ročník

70

Číslo

jaro

Stát

Česká republika

Strany počet

19

URL

https://link.springer.com/article/10.1007/s12223-024-01212-w#citeas

BibTex

@article{BUT193545,
  author="Zuzana {Kroupová} and Eva {Slaninová} and Kateřina {Mrázová} and Vladislav {Krzyžánek} and Kamila {Hrubanová} and Ines {Fritz} and Stanislav {Obruča}",
  title="Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment",
  journal="FOLIA MICROBIOLOGICA",
  year="2024",
  volume="70",
  number="jaro",
  pages="19",
  doi="10.1007/s12223-024-01212-w",
  issn="1874-9356",
  url="https://link.springer.com/article/10.1007/s12223-024-01212-w#citeas"
}