Detail publikace

Comparison of Voltage-Sensitive Dye di-4-ANNEPS Effects in Isolated Hearts of Rat, Guinea Pig, and Rabbit

FIALOVÁ, K. KOLÁŘOVÁ, J. PROVAZNÍK, I. NOVÁKOVÁ, M.

Originální název

Comparison of Voltage-Sensitive Dye di-4-ANNEPS Effects in Isolated Hearts of Rat, Guinea Pig, and Rabbit

Anglický název

Comparison of Voltage-Sensitive Dye di-4-ANNEPS Effects in Isolated Hearts of Rat, Guinea Pig, and Rabbit

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

čeština

Originální abstrakt

Voltage-sensitive dyes (VSDs) are used for recording of monophasic action potentials (MAPs) by optical method in excitable tissues. Their direct effects on the tissue are not completely elucidated. Previously we studied effects of VSD di-4-ANEPPS during staining and washout in Langendorff-perfused guinea pig and rabbit isolated hearts. However, most often used species in basic cardiology is rat. Therefore, we decided to compare the electrophysiological effects of VSD in isolated hearts of rabbit, guinea pig, and rat. Touch-less electrogram was recorded, the heart rate assessed and normalized. The type and incidence of arrhythmias were evaluated. Based on our results - although the procedure of staining with the dye affects electrophysiological properties of the myocardium in all studied species - rat heart may be considered as the most suitable for electrophysiological studies using di-4-ANEPPS.

Anglický abstrakt

Voltage-sensitive dyes (VSDs) are used for recording of monophasic action potentials (MAPs) by optical method in excitable tissues. Their direct effects on the tissue are not completely elucidated. Previously we studied effects of VSD di-4-ANEPPS during staining and washout in Langendorff-perfused guinea pig and rabbit isolated hearts. However, most often used species in basic cardiology is rat. Therefore, we decided to compare the electrophysiological effects of VSD in isolated hearts of rabbit, guinea pig, and rat. Touch-less electrogram was recorded, the heart rate assessed and normalized. The type and incidence of arrhythmias were evaluated. Based on our results - although the procedure of staining with the dye affects electrophysiological properties of the myocardium in all studied species - rat heart may be considered as the most suitable for electrophysiological studies using di-4-ANEPPS.

Klíčová slova

Voltage-sensitive dyes, monophasic action potential, perfused heart

Klíčová slova v angličtině

Voltage-sensitive dyes, monophasic action potential, perfused heart

Autoři

FIALOVÁ, K.; KOLÁŘOVÁ, J.; PROVAZNÍK, I.; NOVÁKOVÁ, M.

Vydáno

26. 9. 2010

Nakladatel

IEEE

Místo

Belfast, Ireland

ISBN

978-1-4244-7319-9

Kniha

Computing in Cardiology

ISSN

0276-6574

Periodikum

Computers in Cardiology

Stát

Spojené státy americké

Strany od

565

Strany do

568

Strany počet

4

BibTex

@inproceedings{BUT34993,
  author="Kateřina {Fialová} and Jana {Kolářová} and Valentine {Provazník} and Marie {Nováková}",
  title="Comparison of Voltage-Sensitive Dye di-4-ANNEPS Effects in Isolated Hearts of Rat, Guinea Pig, and Rabbit",
  booktitle="Computing in Cardiology",
  year="2010",
  journal="Computers in Cardiology",
  pages="565--568",
  publisher="IEEE",
  address="Belfast, Ireland",
  isbn="978-1-4244-7319-9",
  issn="0276-6574"
}