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Project detail
Duration: 01.01.2016 — 31.12.2018
Funding resources
Czech Science Foundation - Standardní projekty
- whole funder (2016-01-01 - 2018-12-31)
On the project
Zrealizujeme extrémně rychlý miniaturní systém pro diferenční skenovací fluorimetrii (DSF). V navrženém systému na čipu vzorek o objemu menším než nanolitr poteče kapilárou s teplotním gradientem. Bílkovina označená fluorescenční značkou se při určité teplotě rozbalí a podle polohy v kapiláře, kde k rozbalení došlo, bude určena i teplota potřebná k rozbalení. Jeden z cílů je vytvořit extrémně rychlou DSF , která bude schopna provést test pod 1 sekundu. Tato metoda je velmi slibná pro rychlé testování a genetická studia. Systém a metoda bude nejprve testována a optimalizována pomocí teploty tání DNA v přítomnosti SYBG-Green I. Bude-li metoda s DNA fungovat budou ověřeny testy s bílkovinami. Bílkoviny budou postupně ohřáty a budeme detekovat změnu teploty jejich rozbalení v důsledku přítomnosti léků, léků, čímž se prokáže vliv léků na stabilitu bílkovin.
Description in EnglishWe will develop an ultrafast miniature system for differential scanning fluorimetry (DFS). The sample with volume below 1 nL will flow through the fabricated microfluidic device inside a capillary with temperature gradient. Fluorescent labeled protein will unfold at certain temperature and based on the location inside the capillary we will be able to determine temperature required for protein unfolding. We will design a system capable of performing this test in less than 1 s. This method is promising for rapid testing as well as genetic studies. First we will test this method using double stranded DNA in presence of SYBR Green I. Once this testing is successfully completed we will verify it with proteins. Proteins will be gradually warmed up and we will detect their unfolding temperature in presence of drugs to determine their influence on protein stability.
KeywordsMikrofluidika; MEMS; diferenční fluorimetrie; výzkum léků; analýza tání; bílkoviny
Key words in EnglishResearch area Microfluidics; MEMS; differential fluorimetry; Research of medicines; analysis of melting; protein
Mark
GA16-11140S
Default language
Czech
People responsible
Hubálek Jaromír, prof. Ing., Ph.D. - principal person responsible
Units
Department of Microelectronics- responsible department (2017-01-10 - not assigned)division-MEL-SIX - beneficiary (2016-01-01 - 2018-12-31)
Results
NEUŽIL P. , AHRBERG C. D., MANZ A. Palm-Sized Device for Point-of-Care Ebola Detection. Analytical Chemistry, 2016, vol. 88, no. 9, p. 4803-4807. ISSN: 1520-6882.Detail
HRDÝ, R.; KYNCLOVÁ, H.; KLEPÁČOVÁ, I.; BARTOŠÍK, M.; NEUŽIL, P. Portable lock-in amplifier-based electrochemical method to measure an array of 64 sensors for point of care applications. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017. 2020. p. 479-480. ISBN: 978-0-69-294183-6.Detail
GABLECH, I.; CAHA, O.; SVATOŠ, V.; PEKÁREK, J.; NEUŽIL, P.; ŠIKOLA, T. Stress-free deposition of [001] preferentially oriented titanium thin film by Kaufman ion-beam source. Thin Solid Films, 2017, vol. 638, no. NA, p. 57-62. ISSN: 0040-6090.Detail
GABLECH, I.; CAHA, O.; SVATOŠ, V.; PRÁŠEK, J.; PEKÁREK, J.; NEUŽIL, P.; ŠIKOLA, T. Preparation of [001] Oriented Titanium Thin Film for MEMS Applications by Kaufman Ion-beam Source. In Proceedings of 9 th International conference Nanocon 2017. 1. Ostrava: Tanger, 2018. p. 117-122. ISBN: 978-80-87294-59-8.Detail
FOHLEROVÁ, Z.; HUBÁLEK, J.; PŘIKRYLOVÁ, K.; POLÁKOVÁ, K. A comparative Study of TiO2 Surfaces with Different Topographies on Osteoblast Behavior. Aveiro, Portugalsko: 2016.Detail
HRDÝ, R.; NEUŽIL, P.; HUBÁLEK, J. Electrochemical Array of Sensors for Point-of-care Applications based on Lock-in Amplification. In PROCEEDINGS OF THE INTERNATIONAL CONFERENCE MODERN ELECTROCHEMICAL METHODS XXXVIII. STRIZOVICKA 19, USTI NAD LABEM, 400 01, CZECH REPUBLIC: LENKA SRSENOVA-BEST SERVIS, 2018. p. 85-89. ISBN: 978-80-905221-6-9.Detail
ZHU, H.; ZHANG, H.; XU, Y.; LAŠŠÁKOVÁ, S.; KORABEČNÁ, M.; NEUŽIL, P. PCR past, present and future. BIOTECHNIQUES, 2020, vol. 69, no. 4, p. 1-10. ISSN: 0736-6205.Detail
PODEŠVA, P.; GABLECH, I.; NEUŽIL, P. Nanostructured Gold Microelectrode Array for Ultrasensitive Detection of Heavy Metal Contamination. Analytical Chemistry, 2018, vol. 90, no. 2, p. 1161-1167. ISSN: 1520-6882.Detail
HRDÝ, R.; KYNCLOVÁ, H.; KLEPÁČOVÁ, I.; BARTOŠÍK, M.; NEUŽIL, P. Portable Lock-in Amplifier-Based Electrochemical Method to Measure an Array of 64 Sensors for Point-of-Care Applications. ANALYTICAL CHEMISTRY, 2017, vol. 86, no. 17, p. 8731-8737. ISSN: 0003-2700.Detail
FENG, J.; FOHLEROVÁ, Z.; LIU, X.; CHANG, H.; NEUŽIL, P. Microfluidic device based on deep reactive ion etching process and its lag effect for single cell capture and extraction. Sensors and Actuators B: Chemical, 2018, vol. 269, no. 1, p. 288-292. ISSN: 0925-4005.Detail
GABLECH, I.; SOMER, J.; FOHLEROVÁ, Z.; SVATOŠ, V.; PEKÁREK, J.; KURDÍK, S.; FENG, J.; FECKO, P.; PODEŠVA, P.; HUBÁLEK, J.; NEUŽIL, P. Fabrication of buried microfluidic channels with observation windows using femtosecond laser photoablation and parylene-C coating. Microfluidics and Nanofluidics, 2018, vol. 22, no. 9, p. 1-7. ISSN: 1613-4990.Detail
Haoqing Zhang, Ying Xu, Zdenka Fohlerova, Honglong Chang, Ciprian Iliescu, Pavel Neuzil. LAMP-on-a-Chip: Revising Microfluidic Platforms for Loop-Mediated DNA Amplification. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2019, no. 5, p. 44-53. ISSN: 0165-9936.Detail
Li, H., Zhanga, H., Xua Y., Tureckova, A., Zahradnik, P., Chang, H., Neuzil, P. Versatile digital polymerase chain reaction chip design, fabrication, and image processing. Sensors and Actuators B: Chemical, 2019, vol. 283, no. 1, p. 677-684. ISSN: 0925-4005.Detail
Castro, ER., Tarn, MD., Ginterová, P., Zhu, H., Xu, Y., Neužil, P. Determination of dynamic contact angles within microfluidic devices. Microfluidics and Nanofluidics, 2018, vol. 22, no. 51, p. 1-11. ISSN: 1613-4982.Detail
Hanliang Zhu, Huanan Li, Haoqing Zhang, Zdenka Fohlerova,Sheng Ni, Levent Yobas, Jaroslav Klempa, Imrich Gablech, Jaromir Hubalek, Honglong Chang, Pavel Neuzil. Heat Transfer Time Determination Based on DNA Melting Curve Analysis. Microfluidics and Nanofluidics, 2020, no. 10, p. 1-7. ISSN: 1613-4982.Detail
ZHANG, H.; LI, H.; ZHU, H.; PEKÁREK, J.; PODEŠVA, P.; CHANG, H.; NEUŽIL, P. Revealing the Secrets of PCR. Sensors and Actuators B: Chemical, 2019, vol. 298, no. NA, p. 1-6. ISSN: 0925-4005.Detail
ZHANG, H.; CHANG, H.; NEUŽIL, P. DEP-on-a-Chip: Dielectrophoresis Applied to Microfluidic Platforms. Micromachines, 2019, vol. 10, no. 6, p. 1-22. ISSN: 2072-666X.Detail
ILIESCU, F.; VRTAČNIK, D.; NEUŽIL, P.; ILIESCU, C. Microfluidic Technology for Clinical Applications of Exosomes. Micromachines, 2019, vol. 10, no. 6, p. 1-29. ISSN: 2072-666X.Detail
LEDNICKÝ, T.; BONYÁR, A. Large Scale Fabrication of Ordered Gold Nanoparticle-Epoxy Surface Nanocomposites and Their Application as Label-Free Plasmonic DNA Biosensors. ACS APPL MATER INTER, 2020, vol. 12, no. 4, p. 4804-4814. ISSN: 1944-8244.Detail
FOHLEROVÁ, Z.; ZHU, H.; HUBÁLEK, J.; NI, S.; PODEŠVA, P.; OTÁHAL, A.; NEUŽIL, P.; YOBAS, L. Rapid Characterization of Biomolecules’ Thermal Stability in Segmented Flow-Through Optofluidic Microsystem. Scientific Reports, 2020, vol. 10, no. 1, p. 1-9. ISSN: 2045-2322.Detail
FOHLEROVÁ, Z.; PODEŠVA, P.; NEUŽIL, P.: Optofluidní čip; Optofluidní mikročip s teplotním gradientem pro určování teplotní stability biomolekul v segmentovaném toku. Ústav mikroelektroniky Vysoké učení technické v Brně. (funkční vzorek)Detail
HRDÝ, R.; FOHLEROVÁ, Z.; SVATOŠ, V.; HUBÁLEK, J.; NEUŽIL, P.: 64-elektrodový čip 2. generace; Elektrochemický 64-mikroelektrodový čip pro biochemickou detekci. Laboratoře LAbSensNano N.066 Technická 10, Brno. (funkční vzorek)Detail
GABLECH, I.; SOMER, J.; FOHLEROVÁ, Z.; KURDÍK, S.; PEKÁREK, J.; FECKO, P.; SVATOŠ, V.; HUBÁLEK, J.; NEUŽIL, P.: Utopené mikrokanály; Utopené mikrokanály pro mikrofluidiku. Laboratoř 0.67 UMEL, VUT, Technická 10, Brno. URL: http://www.umel.feec.vutbr.cz/LabSensNano/products.aspx?id=134. (funkční vzorek)Detail