Project detail

Real-time mapping of cell traction forces

Duration: 01.01.2019 — 31.12.2021

Funding resources

Czech Science Foundation - Juniorské granty

- whole funder (2019-02-18 - 2021-12-31)

On the project

Living cells constantly receive and respond to physical and biochemical signals that can evoke the traction force in cells. This force generated by cells contributes to the regulation of many physiological and pathological processes. Here we propose a technological approach for the measurement of cellular traction force by nanofabrication of a membrane of parylene nanopillars with diameter below 1 µm and the height between 2 µm to 10 µm. The nanopillars will be coated with a layer of thermally growing SiO2. The chemical properties of SiO2 will be simply modulated through the silanization process and plasma treatment. That will enable us to immobilize via covalent crosslinking any polymer include proteins to the pillar surface or to keep the surface non – adhesive for proteins or cells. Our fabrication process also enables us to form in situ a cell culturing well from the remaining silicon. This will greatly simplify the manipulation and overall experimental procedures with cells. The measurement of traction force will be shown on an example of cancer and cardiac cells.

Keywords
traction force; parylene; nanopillars; silanization; proteins; cells; image analysis

Mark

19-04270Y

Default language

English

People responsible

Fohlerová Zdenka, doc. Mgr., Ph.D. - principal person responsible

Units

Central European Institute of Technology BUT
- responsible department (2018-04-09 - 2018-04-09)
Smart Nanodevices
- beneficiary (2019-01-01 - 2021-12-31)

Results

LIU, X.; FECKO, P.; FOHLEROVÁ, Z.; PEKÁREK, J.; KARÁSEK, T.; NEUŽIL, P. Parylene Micropillars Coated with Thermally Grown SiO2. JOURNAL OF VACUUM SCIENCE & TECHNOLOGY B, 2020, vol. 38, no. 1, p. 38-43. ISSN: 2166-2746.
Detail

ZHANG, H.; PEKÁREK, J.; FENG, J.; LIU, X.; Li, H.; ZHU, H.; SVATOŠ, V.; GABLECH, I.; PODEŠVA, P.; NI, S.; YOBAS, L.; NEUŽIL, P. Nanolithography Toolbox - Simplifying the design complexity of microfluidic chips. JOURNAL OF VACUUM SCIENCE & TECHNOLOGY B, 2020, vol. 38, no. 6, p. 1-8. ISSN: 2166-2746.
Detail

BÍLEK, O.; FIALOVÁ, T.; OTÁHAL, A.; ADAM, V.; ŠMERKOVÁ, K.; FOHLEROVÁ, Z. Antibacterial activity of AgNPs-TiO2 nanotubes: influence of different nanoparticle stabilizers. RSC Advances, 2020, vol. 10, no. 72, p. 1-10. ISSN: 2046-2069.
Detail

KOŠELOVÁ, Z.;FOHLEROVÁ, Z.; GABLECH, I.; FECKO, P. High aspect ratio Parylene-C micropillars for cellular force measurement. NANOCON 2021 conference, Brno, Czech Republic, 2021. p. 1 (1 s.).
Detail

CHMELÍKOVÁ, L.; FECKO, P.; CHMELÍK, J.; SKÁCEL, J.; OTÁHAL, A.; FOHLEROVÁ, Z. Demolded hollow high aspect-ratio parylene-C micropillars for real-time mechanosensing applications. Applied Materials Today, 2023, vol. 31, no. 1, p. 1-12. ISSN: 2352-9407.
Detail

PEKÁRKOVÁ, J.; GABLECH, I.; FIALOVÁ, T.; BÍLEK, O.; FOHLEROVÁ, Z. Modifications of Parylene by Microstructures and Selenium Nanoparticles: Evaluation of Bacterial and Mesenchymal Stem Cell Viability. Frontiers in Bioengineering and Biotechnology, 2021, vol. 9, no. 1, p. 1-11. ISSN: 2296-4185.
Detail

FOHLEROVÁ, Z.; GABLECH, I.; OTÁHAL, A.; FECKO, P. SiO2-Decorated Parylene C Micropillars Designed to Probe Cellular Force. Advanced Materials Interfaces, 2021, vol. 8, no. 6, p. 2001897-1 (2001897-8 p.)ISSN: 2196-7350.
Detail

FOHLEROVÁ, Z.; FECKO, P.; GABLECH, I.; KOŠELOVÁ, Z. High aspect ratio Parylene C micropillars formed by molding and ION-BEAM etching method. In NANOCON Conference Proceedings - International Conference on Nanomaterials. TANGER, 2022. p. 1-4. ISBN: 9788088365006.
Detail

FOHLEROVÁ, Z.; GABLECH, I.; OTÁHAL, A.; FECKO, P. SiO2-decorated Parylene C micropillars designed to probe cellular force. SENSORS 2021 conference, Milano, Italy, 2021. p. 1 (1 s.).
Detail

Responsibility: Fohlerová Zdenka, doc. Mgr., Ph.D.