Project is focused on the healing of critical segmental bone defects, which are made during a surgical treatment of cancers, using innovative scaffolds based on titanium. Porous composite scaffolds from titanium alloy Ti-Cu, Ti-Nb and Ti/hydroxyapatite will be prepared. Ti-Cu scaffolds display antibacterial effects and others scaffolds will be developed for improved osteointegration. All the materials are produced by extrusion base additive manufacturing (robocasting), a more cost effective and versatile additive manufacturing method that selective laser melting (SLM), commonly used for the fabrication of Ti-6Al-4V implants. The scaffolds will be tested in cocultures in vitro using human mesenchymal stem cells isolated from bone marrow, Saos-2 cells and endothelial cells for the study of osteogenesis and angiogenesis and THP-1 cells -derived macrophages for the study of immune reactions. Subsequently, the selected scaffolds will be tested in vivo for healing of critical bone defects in a model of rabbit and pig.

Keywords
titanium, composite, scaffold, osteosarcoma, implnat, prosthesis