Detail projektu

Unraveling the role of polyhydroxyalkanoates in Schlegelella thermodepolymerans – promising environmental bacterium for next generation biotechnology

Období řešení: 01.01.2022 — 31.12.2024

Zdroje financování

Grantová agentura České republiky - Standardní projekty

- plně financující (2022-04-26 - 2024-12-31)

O projektu

Schlegelella thermodepolymerans is a thermophilic bacterium that was isolated thanks to capability of polymer degradation. Nevertheless, we have recently identified the extraordinary biotechnological potential of the bacterium within the concept of Next Generation Biotechnology. Apart from polymer degradation, S. thermodepolymerans is also able to produce polyhydroxyalkanoates (PHA), microbial polyesters serving as a storage of carbon and energy and enhancing stress robustness of bacterial cells against numerous environmental stressors. Moreover, PHA are considered to be an ecological alternative to petrochemical polymers. In the proposed project we will in-depth look into PHA metabolism of S. thermodepolymerans especially in the context of stress robustness of the bacterium, we will construct a detailed metabolic model, develop and optimize molecular tools for genome editing and we will also test the employment of the bacterium in the most promising biotechnological applications - production of PHA, biodegradation of polymers and biotransformation/degradation of phenolic compounds.

Klíčová slova
Schlegelella thermodepolymerans; polyhydroxyalkanoates; environmental biotechnology; industrial biotechnology; biodegradation; bioinfromatics; genetic enineering

Označení

GA22-10845S

Originální jazyk

angličtina

Řešitelé

Obruča Stanislav, prof. Ing., Ph.D. - hlavní řešitel
Sedlář Karel, doc. Mgr. Ing., Ph.D. - spoluřešitel

Útvary

Centrum materiálového výzkumu
- odpovědné pracoviště (07.04.2021 - 07.04.2021)
CMV - laboratoř biotechnologií
- odpovědné pracoviště (07.04.2021 - 07.04.2021)
Fakulta chemická
- odpovědné pracoviště (07.04.2021 - 07.04.2021)
Ústav chemie potravin a biotechnologií
- příjemce (01.01.2022 - 31.12.2024)
Ústav biomedicínského inženýrství
- spolupříjemce (01.01.2022 - 31.12.2024)

Výsledky

SEDLÁŘ, K.; MUSILOVÁ, J.; KOUŘILOVÁ, X.; BEZDÍČEK, M.; OBRUČA, S. Diversity of Schlegelella thermodepolymerans Revealed with Nanopore Sequencing. In Proceedings of the 9th International Conference on Chemical Technology. Prague: Czech Society of Industrial Chemistry, 2022. p. 373-378. ISBN: 978-80-88307-11-2.
Detail

HEŘMÁNKOVÁ, K.; KOUŘILOVÁ, X.; BUCHTÍKOVÁ, I.; OBRUČA, S.; SEDLÁŘ, K. PHA-Related Regulatory Small RNAs in Caldimonas Thermodepolymerans. 9th Meeting on Chemistry & Life for Sustainable Future Book of Abstracts. 1. 2024. p. 42-42.
Detail

MUSILOVÁ, J.; KOUŘILOVÁ, X.; HEŘMÁNKOVÁ, K.; BEZDÍČEK, M.; OBRUČA, S.; SEDLÁŘ, K. Comparative genomic analysis of two Schlegelella species brings novel insights into polyhydroxyalkanoates production. 10th International Chemical-Technological Conference (ICCT), Mikulov, Czechia. 2023.
Detail

MUSILOVÁ, J.; KOUŘILOVÁ, X.; BEZDÍČEK, M.; OBRUČA, S.; SEDLÁŘ, K. Comparison of short and long reads in structural and functional annotation of non-model bacteria. 2022. p. 11-11.
Detail

ŘEHÁKOVÁ, V.; PERNICOVÁ, I.; OBRUČA, S. Thermophilic Gram-positive bacteria of the genus Aneurinibacillus: Unique producers of various PHA copolymers. Studentská odborná konference Chemie je život 2022; Sborník abstraktů. Brno: Vysoké učení technické v Brně, Fakulta chemická, 2023. p. 44 ( p.)ISBN: 978-80-214-6117-8.
Detail

MUSILOVÁ, J.; VAFEK, Z.; HEŘMÁNKOVÁ, K.; KOUŘILOVÁ, X.; PERNICOVÁ, I.; OBRUČA, S.; PUNYIA, B.; HELIKAR, T.; SEDLÁŘ, K. Introducing Augusta, a Python package for inferring Gene Regulatory and Boolean Networks from RNA-Seq data, and its application to the Caldimonas thermodepolymerans DSM 15344T. 2023.
Detail

HRABALOVÁ, V.; OPIAL, T.; OBRUČA, S. Biotransformation of ferulic acid by using bacteria Caldimonas thermodepolymerans. Abstracts 16th International Congress "Thermophiles-2023". 1. Wales, United Kingdom: Bangor University, 2023. p. 11-11.
Detail

ŠEDRLOVÁ, Z.; MRÁZOVÁ, K.; HRUBANOVÁ, K.; KRZYZANEK, V.; OBRUČA, S. Characterization of biotechnologically relevant bacterial cells. Hradec Králové: IMAGING PRINCIPLES OF LIFE 2023, 2023. p. 48-48.
Detail

MUSILOVÁ, J.; KOUŘILOVÁ, X.; HEŘMÁNKOVÁ, K.; BEZDÍČEK, M.; IEREMENKO, A.; DVOŘÁK, P.; OBRUČA, S.; SEDLÁŘ, K. Genomic and Phenotypic Comparison of Polyhydroxyalkanoates Producing Strains of genus Caldimonas/Schlegelella. Computational and Structural Biotechnology Journal, 2023, vol. 21, no. November, p. 5372-5381. ISSN: 2001-0370.
Detail

PERNICOVÁ, I.; ŘEHÁKOVÁ, V.; KOUŘILOVÁ, X.; SEDLÁČEK, P.; OBRUČA, S. Using Of “Hot” Genus Aneurinibacillus To Produce Unique Polyhydroxyalkanoate copolymers. European Symposium on Biopolymers Book of Abstracts. Brno: VUT, 2023. p. 109-109.
Detail

ŘEHÁKOVÁ, V.; PERNICOVÁ, I.; SEDLÁČEK, P.; KALINA, M.; OBRUČA, S. PRODUKCE UNIKÁTNÍCH PHA KOPOLYMERŮ TERMOFILNÍMI GRAMPOZITIVNÍMI BAKTERIEMI RODU ANEURINIBACILLUS. Book of Abstracts. 10th International Conference on Chemical Technology. 1. Praha: AMCA, spol. s.r.o., 2023. s. 33-33. ISBN: 978-80-88214-40-3.
Detail

KOUŘILOVÁ, X.; PERNICOVÁ, I.; MUSILOVÁ, J.; SEDLÁŘ, K.; DVOŘÁK, P.; OBRUČA, S. Caldimonas thermodepolymerans - PHA producer with unique properties. 16th International Congress "Thermophiles-2023. Wales, United Kingdom: Bangor University, 2023. p. P15 ( p.)
Detail

KOUŘILOVÁ, X.; PERNICOVÁ, I.; MUSILOVÁ, J.; SEDLÁŘ, K.; DVOŘÁK, P.; OBRUČA, S. Thermophilic PHA Producer sui generis - Caldimonas thermodepolymerans. European Symposium on Biopolymers. Brno: 2023. p. L32 ( p.)
Detail

MUSILOVÁ, J.; VAFEK, Z.; PUNYIA, B.; ZIMMER, R.; HELIKAR, T.; SEDLÁŘ, K. Augusta: From RNA-Seq to Gene Regulatory Networks and Boolean Models. Computational and Structural Biotechnology Journal, 2024, vol. 23, no. December 2024, p. 783-790. ISSN: 2001-0370.
Detail

HRUBANOVÁ, K.; SIKOROVÁ, P.; MRÁZOVÁ, K.; NEBESÁŘOVÁ, J.; OBRUČA, S.; KRZYŽÁNEK, V. Morphological Study of PHA Producing Bacteria. In Mictoscopy and Microanalysis. Microscopy and Microanalysis. Oxford: Oxford Academic, 2023. p. 883-884. ISSN: 1435-8115.
Detail

HRABALOVÁ, V.; OPIAL, T.; MUSILOVÁ, J.; SEDLÁŘ, K.; OBRUČA, S. Biotransformation of ferulic acid into vanillyl alcohol and vanillic acid employing thermophilic bacterium Caldimonas thermodepolymerans. ENZYME AND MICROBIAL TECHNOLOGY, 2024, vol. 179, no. 9, p. 1-6. ISSN: 0141-0229.
Detail

HRABALOVÁ, V.; OPIAL, T.; MUSILOVÁ, J.; SEDLÁŘ, K.; POŘÍZKA, J.; DIVIŠ, P.; OBRUČA, S. Conversion of Ferulic Acid by Extremophilic Bacteria. Book of Abstracts. Brno: 2024.
Detail

HEŘMÁNKOVÁ, K.; KOUŘILOVÁ, X.; BUCHTÍKOVÁ, I.; OBRUČA, S.; SEDLÁŘ, K. Small RNA Analysis in Caldimonas thermodepolymerans Provides Insight into Non-Coding Regulatory Mechanisms. 11th International Conference on Chemical Technology Book of Abstracts. 1. 2024. p. 200-200. ISBN: 978-80-88214-48-9.
Detail

ŘEHÁKOVÁ, V.; PERNICOVÁ, I.; KOUŘILOVÁ, X.; SEDLÁČEK, P.; MUSILOVÁ, J.; SEDLÁŘ, K.; KOLLER, M.; KALINA, M.; OBRUČA, S. Biosynthesis of versatile PHA copolymers by thermophilic members of the genus Aneurinibacillus. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2023, vol. 225, no. 225, p. 1588-1598. ISSN: 0141-8130.
Detail

Odpovědnost: Obruča Stanislav, prof. Ing., Ph.D.