The project focuses on the solution of a complex system of separation of protein samples and subsequent scanning of electroanalytical signals in microfluidic configurations using optimized microelectrode fields (MEAs). The combination of MEAs with electronic lock-in amplifiers allows very rapid protein analysis with low detection limits using new types of pulse methods while maintaining reproducibility comparable to laboratory potentiostats. Protein-like intracellular and free-flowing proteins work as potential markers for a variety of diseases. Their separation from blood samples will bring progress in the possibility of early detection of, e.g., cancer. The separation of blood samples and the use of MEAs on the chip itself is up to date in protein assays, as they eliminate some of the disadvantages of standard electrode systems, such as low achievable rate determination of large numbers of samples and insufficient detection limits. This portable chip can then be configured for a wide range of Lab-On-Chip applications.

Keywords
microelectrode array:lab-on-chip;microfluidic chip;biosensor;lock-in amplifier;glutathione

20-30129Y

English

Hrdý Radim, Ing., Ph.D. - principal person responsible

Smart Nanodevices
- responsible department (5.4.2019 - not assigned)
Smart Nanodevices
- beneficiary (1.1.2020 - 31.12.2022)

MIDLIK, S.; GABLECH, I.; GOLEŇA, M.; BRODSKÝ, J.; SCHMORANZER, D. Parylene-bonded micro-fluidic channels for cryogenic experiments at superfluid He-4 temperatures. Review of Scientific Instruments, 2024, vol. 95, no. 3, ISSN: 0034-6748.
Detail

GABLECH, I.; MIGLIACCIO, L.; BRODSKÝ, J.; HAVLÍČEK, M.; PODEŠVA, P.; HRDÝ, R.; EHLICH, J.; GRYSZEL, M.; GLOWACKI, E. High-Conductivity Stoichiometric Titanium Nitride for Bioelectronics. Advanced Electronic Materials, 2023, vol. 9, no. 4, p. 1-11. ISSN: 2199-160X.
Detail

EHLICH, J.; MIGLIACCIO, L.; SAHALIANOV, I.; NIKIĆ, M.; BRODSKÝ, J.; GABLECH, I.; VU, X.; INGEBRANDT, S.; GLOWACKI, E. Direct measurement of oxygen reduction reactions at neurostimulation electrodes. Journal of Neural Engineering, 2022, vol. 19, no. 3, p. 036045 ( p.)ISSN: 1741-2552.
Detail

Responsibility: Hrdý Radim, Ing., Ph.D.