Paper-based microfluidic analytical devices (µ-PADs) significantly advance the options of rapid and low-cost portable analysis. Instrumentation-free readout based on distance-based detection enhances the value of µ-PADs due to their extremely low cost, and simplicity of use without any instrumentation required. Selective reagents have to be immobilised on the µ-PAD to achieve formation of an accurate reaction boundary allowing distance-based detection, but so far only very limited range of immobilised reagent have been used. Herein we propose new ways of reagent immobilisation, thus broadening the range of reagents that can be used. We propose to utilise electrostatic interaction on µ-PADs with paper substrate modified to carry ionic charges, and explore other approaches including paper-embedded nano- or microparticles. Finally, integrated sample preparation compatible with distance-based detection µ-PADs will be studied for the determination of selected (bio)analytes in samples with various types of matrices.

Keywords
Paperfluidic-based analysis;analytical devices;portable analysis;instrumentation-free detection

19-02108S

English

Macka Miroslav, doc. RNDr., Ph.D. - principal person responsible
Nejdl Lukáš, Ing., Ph.D. - fellow researcher
Rýpar Tomáš, Bc. - fellow researcher
Vaculovičová Markéta, doc. Mgr., Ph.D. - fellow researcher

Central European Institute of Technology BUT
- responsible department (5.4.2018 - 11.4.2018)
Smart Nanodevices
- responsible department (11.4.2018 - not assigned)
Smart Nanodevices
- beneficiary (1.1.2019 - 31.12.2021)

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Detail

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KHUNKITCHAI, N.; NUCHTAVORN, N.; RÝPAR, T.; VLČNOVSKÁ, M.; NEJDL, L.; VACULOVIČOVÁ, M.; MACKA, M. UV-light-actuated in-situ preparation of paper@ZnCd quantum dots for paper-based enzymatic nanoreactors. CHEMICAL ENGINEERING JOURNAL, 2022, vol. 428, no. 132508, p. 1-5. ISSN: 1385-8947.
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VODOVÁ, M.; NEJDL, L.; PAVELICOVÁ, K.; ZEMÁNKOVÁ, K.; RÝPAR, T.; ŠTĚRBOVÁ, D.; BEZDĚKOVÁ, J.; NUCHTAVORN, N.; MACKA, M.; ADAM, V.; VACULOVIČOVÁ, M. Detection of pesticides in food products using paper-based devices by UV-induced fluorescence spectroscopy combined with molecularly imprinted polymers. FOOD CHEMISTRY, 2022, vol. 380, no. 132141, p. 1-9. ISSN: 0308-8146.
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ASSI, N.; NEJDL, L.; ZEMÁNKOVÁ, K.; PAVELICOVÁ, K.; BEZDĚKOVÁ, J.; MACKA, M.; ADAM, V.; VACULOVIČOVÁ, M. UV-induced Zn:Cd/S quantum dots in-situ formed in the presence of thiols for sensitive and selective fluorescence detection of thiols. Scientific Reports, 2021, vol. 11, no. 1, p. 1-11. ISSN: 2045-2322.
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RÝPAR, T.; ADAM, V.; VACULOVIČOVÁ, M.; MACKA, M. Paperfluidic devices with a selective molecularly imprinted polymer surface for instrumentation-free distance-based detection of protein biomarkers. Sensors and Actuators B: Chemical, 2021, vol. 341, no. 129999, p. 1-10. ISSN: 0925-4005.
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CHUTVIRASAKUL, B.; NUCHTAVORN, N.; MACKA, M.; SUNTORNSUK, L. Distance-based paper device using polydiacetylene liposome as a chromogenic substance for rapid and in-field analysis of quaternary ammonium compounds. Analytical and Bioanalytical Chemistry, 2020, vol. 412, no. 13, p. 3221-3230. ISSN: 1618-2642.
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Responsibility: Macka Miroslav, doc. RNDr., Ph.D.