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Detail projektu
Období řešení: 01.01.2019 — 31.12.2021
Zdroje financování
Grantová agentura České republiky - Standardní projekty
- plně financující (2019-02-18 - 2021-12-31)
O projektu
Paper-based microfluidic analytical devices (µ-PADs) significantly advance the options of rapid and low-cost portable analysis. Instrumentation-free readout based on distance-based detection enhances the value of µ-PADs due to their extremely low cost, and simplicity of use without any instrumentation required. Selective reagents have to be immobilised on the µ-PAD to achieve formation of an accurate reaction boundary allowing distance-based detection, but so far only very limited range of immobilised reagent have been used. Herein we propose new ways of reagent immobilisation, thus broadening the range of reagents that can be used. We propose to utilise electrostatic interaction on µ-PADs with paper substrate modified to carry ionic charges, and explore other approaches including paper-embedded nano- or microparticles. Finally, integrated sample preparation compatible with distance-based detection µ-PADs will be studied for the determination of selected (bio)analytes in samples with various types of matrices.
Klíčová slovaPaperfluidic-based analysis;analytical devices;portable analysis;instrumentation-free detection
Označení
19-02108S
Originální jazyk
angličtina
Řešitelé
Macka Miroslav, doc. RNDr., Ph.D. - hlavní řešitelNejdl Lukáš, Ing., Ph.D. - spoluřešitelRýpar Tomáš, Bc. - spoluřešitelVaculovičová Markéta, doc. Mgr., Ph.D. - spoluřešitel
Útvary
Chytré nanonástroje- příjemce (01.01.2019 - 31.12.2021)Středoevropský technologický institut- odpovědné pracoviště (05.04.2018 - 05.04.2018)Středoevropský technologický institut VUT- odpovědné pracoviště (05.04.2018 - 11.04.2018)
Výsledky
CHUTVIRASAKUL, B.; NUCHTAVORN, N.; MACKA, M.; SUNTORNSUK, L. Distance-based paper device using polydiacetylene liposome as a chromogenic substance for rapid and in-field analysis of quaternary ammonium compounds. Analytical and Bioanalytical Chemistry, 2020, vol. 412, no. 13, p. 3221-3230. ISSN: 1618-2642.Detail
RÝPAR, T.; ADAM, V.; VACULOVIČOVÁ, M.; MACKA, M. Paperfluidic devices with a selective molecularly imprinted polymer surface for instrumentation-free distance-based detection of protein biomarkers. Sensors and Actuators B: Chemical, 2021, vol. 341, no. 129999, p. 1-10. ISSN: 0925-4005.Detail
ASSI, N.; NEJDL, L.; ZEMÁNKOVÁ, K.; PAVELICOVÁ, K.; BEZDĚKOVÁ, J.; MACKA, M.; ADAM, V.; VACULOVIČOVÁ, M. UV-induced Zn:Cd/S quantum dots in-situ formed in the presence of thiols for sensitive and selective fluorescence detection of thiols. Scientific Reports, 2021, vol. 11, no. 1, p. 1-11. ISSN: 2045-2322.Detail
ASSI, N.; RÝPAR, T.; MACKA, M.; ADAM, V.; VACULOVIČOVÁ, M. Microfluidic paper-based fluorescence sensor for L-homocysteine using a molecularly imprinted polymer and in situ-formed fluorescent quantum dots. TALANTA, 2023, vol. 255, no. 124185, ISSN: 1873-3573.Detail
KHUNKITCHAI, N.; NUCHTAVORN, N.; RÝPAR, T.; VLČNOVSKÁ, M.; NEJDL, L.; VACULOVIČOVÁ, M.; MACKA, M. UV-light-actuated in-situ preparation of paper@ZnCd quantum dots for paper-based enzymatic nanoreactors. CHEMICAL ENGINEERING JOURNAL, 2022, vol. 428, no. 132508, p. 1-5. ISSN: 1385-8947.Detail
NUCHTAVORN, N.; RÝPAR, T.; NEJDL, L.; VACULOVIČOVÁ, M.; MACKA, M. Distance-based detection in analytical flow devices: from gas detection tubes to microfluidic chips and microfluidic paper-based analytical devices. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2022, vol. 150, no. 116581, p. 1-13. ISSN: 0165-9936.Detail
VODOVÁ, M.; NEJDL, L.; PAVELICOVÁ, K.; ZEMÁNKOVÁ, K.; RÝPAR, T.; ŠTĚRBOVÁ, D.; BEZDĚKOVÁ, J.; NUCHTAVORN, N.; MACKA, M.; ADAM, V.; VACULOVIČOVÁ, M. Detection of pesticides in food products using paper-based devices by UV-induced fluorescence spectroscopy combined with molecularly imprinted polymers. FOOD CHEMISTRY, 2022, vol. 380, no. 132141, p. 1-9. ISSN: 0308-8146.Detail